sFRP-2 Antibody

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Western Blot: sFRP-2 Antibody [NB110-74715] - Analysis of sFRP-2 in Human Duodenum lysate (35ug protein in RIPA buffer) using this antibody at 0.1 ug/ml. Primary incubation was 1 hour. Detected by chemiluminescence.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, PEP-ELISA
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

sFRP-2 Antibody Summary

Immunogen
Peptide with sequence C-KRWQKGQREFKR corresponding to C-Terminus according to NP_003004.1.
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
SFRP2
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1 - 3 ug/ml
  • Peptide ELISA Detection limit 1:32000
Application Notes
WB: Approx. 38 kDa band observed in human duodenum lysates (calculated MW of 33.5 kDa band according to NP_003004.1).
Control
sFRP-2 Lysate (NBL1-15884)
Publications
Read Publication using NB110-74715.

Reactivity Notes

Predicted cross-reactivity based on sequence identity: Mouse, Rat, Canine.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Tris (pH 7.3), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for sFRP-2 Antibody

  • FRP2
  • FRP-2secreted apoptosis related protein 1
  • SARP-1
  • SARP1sFRP-2
  • SDF-5
  • Secreted apoptosis-related protein 1
  • secreted frizzled-related protein 2
  • sFRP2
  • sFRP-2

Background

This gene encodes a member of the SFRP family that contains a cysteine-rich domain homologous to the putative Wnt-binding site of Frizzled proteins. SFRPs act as soluble modulators of Wnt signaling. Methylation of this gene is a potential marker for the presence of colorectal cancer. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for sFRP-2 Antibody (NB110-74715)(1)

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Product General Protocols

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Video Protocols

WB Video Protocol

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Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics Tool for sFRP-2 Antibody (NB110-74715)

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Bioinformatics

Gene Symbol SFRP2
Entrez
Uniprot