SFPQ Antibody (JU33-99)

Western Blot: SFPQ Antibody (JU33-99) [NBP2-75664] - Analysis of SFPQ on different lysates using anti-SFPQ antibody at 1/1,000 dilution.Positive control:Lane 1: K562 Lane 2: DaudiLane 3: Mouse testis Lane 4: Mouse lung

Immunohistochemistry-Paraffin: SFPQ Antibody (JU33-99) [NBP2-75664] - Analysis of paraffin-embedded rat brain tissue using anti-SFPQ antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: SFPQ Antibody (JU33-99) [NBP2-75664] - Analysis of paraffin-embedded human tonsil tissue using anti-SFPQ antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: SFPQ Antibody (JU33-99) [NBP2-75664] - Analysis of paraffin-embedded human liver cancer tissue using anti-SFPQ antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: SFPQ Antibody (JU33-99) [NBP2-75664] - Analysis of paraffin-embedded human kidney tissue using anti-SFPQ antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: SFPQ Antibody (JU33-99) [NBP2-75664] - Analysis of paraffin-embedded mouse testis tissue using anti-SFPQ antibody. Counter stained with hematoxylin.

Product Discontinued

SFPQ Antibody (JU33-99) Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Synthetic peptide within Human SFPQ aa 658-707 / 707. (SwissProt: P23246 Human; SwissProt: Q8VIJ6 Mouse)
• Localization: Nucleus. Cytoplasm.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: SFPQ
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:10-1:50
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Western Blot 1:500-1:2000
• Theoretical MW: 100 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: TBS (pH7.4), 0.05% BSA, 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for SFPQ Antibody (JU33-99)

• 100 kDa DNA-pairing protein
• DNA-binding p52/p100 complex, 100 kDa subunit
• hPOMp100
• polypyrimidine tract binding protein associated
• polypyrimidine tract-binding protein-associated splicing factor
• Polypyrimidine tract-binding protein-associated-splicing factor
• PSFPOMP100
• PTB-associated splicing factor
• PTB-associated-splicing factor
• splicing factor proline/glutamine rich (polypyrimidine tract binding proteinassociated)
• splicing factor proline/glutamine rich (polypyrimidine tract-bindingprotein-associated)
• splicing factor proline/glutamine-rich
• splicing factor, proline- and glutamine-rich

Background

PSF/SFPQ is a multifunctional protein know to participate in a variety of nuclear processes and may function to link transcription and pre-mRNA processing. PSF/SFPQ bears an RNA recognition motif (RRM) further indicating its role in post-transcriptional processes. PSF has been shown to associate with p54nrb/NonO to modulate androgen receptor-mediated transcription. Additionally, it has recently been show to associate with XRN2 and p54nrb/NonO to facilitate pre-mRNA 3' processing and transcription termination. Alternate names for PSF/SFPQ include polypyrimidine tract-binding protein-associated-splicing factor, PTB-associated-splicing factor, splicing factor proline-and glutamine-rich, and POMP100.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: SFPQ