SF3B4 Antibody

Western Blot: SF3B4 Antibody [NBP2-20326] - Sample (30 ug of whole cell lysate) A: NIH-3T3 B: JC C: BCL-1 10% SDS PAGE gel, diluted at 1:1000.

Western Blot: SF3B4 Antibody [NBP2-20326] - Sample (30 ug of whole cell lysate) A: 293T B: A431 C: Jurkat 10% SDS PAGE gel, diluted at 1:1000.

Western Blot: SF3B4 Antibody [NBP2-20326] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with SF3B4 antibody (NBP2-20326) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

• Reactivity: Hu, Mu, Rt, Bv, RM, Ze
• Applications: WB
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

SF3B4 Antibody Summary

• Immunogen: Recombinant protein encompassing a sequence within the center region of human SF3B4. The exact sequence is proprietary.
• Localization: Nucleus
• Predicted Species: Rat (100%), Bovine (100%), Rhesus Macaque (100%), Zebrafish (96%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: SF3B4
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:500-1:3000
• Theoretical MW: 44 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Xenopus laevis (88%).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7), 20% Glycerol, 1% BSA
• Preservative: 0.01% Thimerosal
• Purity: Antigen Affinity-purified

Alternate Names for SF3B4 Antibody

• Hsh49
• pre-mRNA splicing factor SF3b 49 kDa subunit
• Pre-mRNA-splicing factor SF3b 49 kDa subunit
• SAP 49
• SAP49MGC10828
• SF3b49
• SF3b50
• spliceosomal protein
• spliceosome-associated protein (U2 snRNP)
• Spliceosome-associated protein 49
• splicing factor 3B subunit 4
• splicing factor 3b, subunit 4, 49kD
• splicing factor 3b, subunit 4, 49kDa

Background

This gene encodes one of four subunits of the splicing factor 3B. The protein encoded by this gene cross-links to a region in the pre-mRNA immediately upstream of the branchpoint sequence in pre-mRNA in the prespliceosomal complex A. It also may be involved in the assembly of the B, C and E spliceosomal complexes. In addition to RNA-binding activity, this protein interacts directly and highly specifically with subunit 2 of the splicing factor 3B. This protein contains two N-terminal RNA-recognition motifs (RRMs), consistent with the observation that it binds directly to pre-mRNA. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Bioinformatics

• Gene Symbol: SF3B4
• Uniprot:
  • Q15427(Human)