SF3B2 Antibody (5D2)

Images

 
Western Blot: SF3B2 Antibody (5D2) [H00010992-M01] - SF3B2 monoclonal antibody (M01), clone 5D2 Analysis of SF3B2 expression in Hela S3 NE.
Immunocytochemistry/ Immunofluorescence: SF3B2 Antibody (5D2) [H00010992-M01] - Analysis of monoclonal antibody to SF3B2 on HeLa cell. Antibody concentration 60 ug/ml.
Immunohistochemistry-Paraffin: SF3B2 Antibody (5D2) [H00010992-M01] - Analysis of monoclonal antibody to SF3B2 on formalin-fixed paraffin-embedded human kidney. Antibody concentration 6 ug/ml.
Western Blot: SF3B2 Antibody (5D2) [H00010992-M01] - SF3B2 monoclonal antibody (M01), clone 5D2. Analysis of SF3B2 expression in PC-12.
Western Blot: SF3B2 Antibody (5D2) [H00010992-M01] - SF3B2 monoclonal antibody (M01), clone 5D2. Analysis of SF3B2 expression in NIH/3T3.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ELISA, ICC/IF, IHC, IHC-P
Clone
5D2
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

SF3B2 Antibody (5D2) Summary

Immunogen
SF3B2 (NP_006833, 592 a.a. ~ 646 a.a) partial recombinant protein with GST tag.YEGKEFETRLKEKKPGDLSDELRISLGMPVGPNAHKVPPPWLIAMQRYGPPPSY
Specificity
SF3B2 (5D2)
Isotype
IgG2a Kappa
Clonality
Monoclonal
Host
Mouse
Gene
SF3B2
Purity
IgG purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500
  • ELISA
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
Application Notes
Antibody reactive against cell lysate and recombinant protein for Western Blot. Has also been used for immunofluoresence, immunohistochemistry (paraffin), and ELISA.
Positive Control
HeLa Lysate (NB800-PC9)

Reactivity Notes

Mouse, rat, Human.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.4)
Preservative
No Preservative
Purity
IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for SF3B2 Antibody (5D2)

  • Cus1
  • pre-mRNA splicing factor SF3b 145 kDa subunit
  • Pre-mRNA-splicing factor SF3b 145 kDa subunit
  • SAP145SAP 145
  • SF3b1
  • SF3B145
  • SF3b150
  • spliceosome associated protein 145
  • Spliceosome-associated protein 145
  • splicing factor 3B subunit 2
  • splicing factor 3b, subunit 2, 145kD
  • splicing factor 3b, subunit 2, 145kDa

Background

This gene encodes subunit 2 of the splicing factor 3b protein complex. Splicing factor 3b, together with splicing factor 3a and a 12S RNA unit, forms the U2 small nuclear ribonucleoproteins complex (U2 snRNP). The splicing factor 3b/3a complex binds pre-mRNA upstream of the intron's branch site in a sequence-independent manner and may anchor the U2 snRNP to the pre-mRNA. Splicing factor 3b is also a component of the minor U12-type spliceosome. Subunit 2 associates with pre-mRNA upstream of the branch site at the anchoring site. Subunit 2 also interacts directly with subunit 4 of the splicing factor 3b complex. Subunit 2 is a highly hydrophilic protein with a proline-rich N-terminus and a glutamate-rich stretch in the C-terminus.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol SF3B2
Entrez
OMIM
Uniprot