SF3A1 Antibody

Product Discontinued

SF3A1 Antibody Summary

• Immunogen: The immunogen recognized by this antibody maps to a region between residue 350 and 400 of human splicing factor 3a, subunit 1, 120kDa using the numbering given in entry NP_005868.1 (GeneID 10291).
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: SF3A1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Immunoprecipitation 1:10-1:500
  • Western Blot 1:2000-1:10000
• Control:

  SF3A1 Overexpression Lysate

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: TBS and 0.1% BSA
• Preservative: 0.09% Sodium Azide
• Concentration: 0.2 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for SF3A1 Antibody

• pre-mRNA processing 21
• pre-mRNA splicing factor SF3a (120 kDa subunit)
• Prp21
• PRPF21
• SAP 114
• SAP114splicing factor 3a, subunit 1, 120kD
• SF3a120
• spliceosome associated protein 114
• Spliceosome-associated protein 114
• splicing factor 3 subunit 1
• splicing factor 3A subunit 1
• splicing factor 3a, subunit 1, 120kDa

Background

This gene encodes subunit 1 of the splicing factor 3a protein complex. The splicing factor 3a heterotrimer includes subunits 1, 2 and 3 and is necessary for the in vitro conversion of 15S U2 snRNP into an active 17S particle that performs pre-mRNA splicing. Subunit 1 belongs to the SURP protein family; named for the SURP (also called SWAP or Suppressor-of-White-APricot) motifs that are thought to mediate RNA binding. Subunit 1 has tandemly repeated SURP motifs in its amino-terminal half while its carboxy-terminal half contains a proline-rich region and a ubiquitin-like domain. Binding studies with truncated subunit 1 derivatives demonstrated that the two SURP motifs are necessary for binding to subunit 3 while contacts with subunit 2 may occur through sequences carboxy-terminal to the SURP motifs. Alternative splicing results in multiple transcript variants encoding different isoforms.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: SF3A1
• Entrez:
  • 10291(Human)
• Uniprot:
  • NP_005868.1(Human)