SF3A1 Antibody (OTI3F4) [mFluor Violet 450 SE]

• Reactivity: Hu, Mu, Rt
• Applications: WB, IHC
• Clone: OTI3F4
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: mFluor Violet 450 SE

SF3A1 Antibody (OTI3F4) [mFluor Violet 450 SE] Summary

• Immunogen: Human recombinant protein fragment corresponding to amino acids 249-568 of human SF3A1 (NP_005868) produced in E.coli.
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: SF3A1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Western Blot
• Application Notes: Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: 50mM Sodium Borate
• Preservative: 0.05% Sodium Azide
• Purity: Immunogen affinity purified

Notes

mFluor(TM) is a trademark of AAT Bioquest, Inc. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Alternate Names for SF3A1 Antibody (OTI3F4) [mFluor Violet 450 SE]

• pre-mRNA processing 21
• pre-mRNA splicing factor SF3a (120 kDa subunit)
• Prp21
• PRPF21
• SAP 114
• SAP114splicing factor 3a, subunit 1, 120kD
• SF3a120
• spliceosome associated protein 114
• Spliceosome-associated protein 114
• splicing factor 3 subunit 1
• splicing factor 3A subunit 1
• splicing factor 3a, subunit 1, 120kDa

Background

SF3A1 encodes subunit 1 of the splicing factor 3a protein complex. The splicing factor 3a heterotrimer includes subunits 1, 2 and 3 and is necessary for the in vitro conversion of 15S U2 snRNP into an active 17S particle that performs pre-mRNA splicing. Subunit 1 belongs to the SURP protein family; named for the SURP (also called SWAP or Suppressor-of-White-APricot) motifs that are thought to mediate RNA binding. Subunit 1 has tandemly repeated SURP motifs in its amino-terminal half while its carboxy-terminal half contains a proline-rich region and a ubiquitin-like domain. Binding studies with truncated subunit 1 derivatives demonstrated that the two SURP motifs are necessary for binding to subunit 3 while contacts with subunit 2 may occur through sequences carboxy-terminal to the SURP motifs. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: SF3A1