SF2 Antibody (JB40-76)

Western Blot: SF2 Antibody (JB40-76) [NBP2-75663] - Analysis of SF2 on different lysates using anti-SF2 antibody at 1/500 dilution.Positive control:Lane 1: Mouse heart Lane 2: Mouse liverLane 3: K562

Immunocytochemistry/Immunofluorescence: SF2 Antibody (JB40-76) [NBP2-75663] - Staining SF2 in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: SF2 Antibody (JB40-76) [NBP2-75663] - Analysis of paraffin-embedded rat epididymis tissue using anti-SF2 antibody. Counter stained with hematoxylin.

Flow Cytometry: SF2 Antibody (JB40-76) [NBP2-75663] - Analysis of K562 cells with SF2 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.

Immunohistochemistry-Paraffin: SF2 Antibody (JB40-76) [NBP2-75663] - Analysis of paraffin-embedded human lung cancer tissue using anti-SF2 antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: SF2 Antibody (JB40-76) [NBP2-75663] - Analysis of paraffin-embedded human tonsil tissue using anti-SF2 antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: SF2 Antibody (JB40-76) [NBP2-75663] - Analysis of paraffin-embedded human breast tissue using anti-SF2 antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: SF2 Antibody (JB40-76) [NBP2-75663] - Analysis of paraffin-embedded mouse colon tissue using anti-SF2 antibody. Counter stained with hematoxylin.

Product Discontinued

SF2 Antibody (JB40-76) Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Synthetic peptide within Human SF2 aa 1-50 / 248. (SwissProt: Q07955 Human; SwissProt: Q6PDM2 Mouse; SwissProt: D4A9L2 Rat)
• Localization: Cytoplasm, Nucleus, Spliceosome.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: SRSF1
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:50-1:100
  • Immunocytochemistry/ Immunofluorescence 1:50
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Western Blot 1:500-1:1000
• Theoretical MW: 28 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: TBS (pH7.4), 0.05% BSA, 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for SF2 Antibody (JB40-76)

• Alternative-splicing factor 1
• ASFpre-mRNA-splicing factor SF2, P33 subunit
• MGC5228
• serine/arginine-rich splicing factor 1
• SF2FLJ53078
• SF2p33
• SFRS1splicing factor 2
• Splicing factor, arginine/serine-rich 1ASF-1
• SR splicing factor 1
• SRp30a

Background

SF2 encodes a member of the arginine/serine-rich splicing factor protein family, and functions in both constitutive and alternative pre-mRNA splicing. The protein binds to pre-mRNA transcripts and components of the spliceosome, and can either activate or repress splicing depending on the location of the pre-mRNA binding site. The protein's ability to activate splicing is regulated by phosphorylation and interactions with other splicing factor associated proteins. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: SRSF1