SESN2 Antibody

Western Blot: SESN2 Antibody [NBP1-44989] - Analysis using the FITC conjugate of NBP1-44989. Detection of SESN2 antibody at 1:500 dilution was probed withSESN2 Positive Control sample. MW of SESN2 is approximately 54.6kDa

• Reactivity: Hu, Mu, Rt
• Applications: WB, DB, ELISA
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: .68 mg/ml

SESN2 Antibody Summary

• Description: Novus Biologicals Rabbit SESN2 Antibody (NBP1-44989) is a polyclonal antibody validated for use in WB and ELISA. Anti-SESN2 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Synthetic peptide taken within amino acid region 1-80 on SESN2 protein.
• Localization: Cytoplasm, Nucleus
• Specificity: This antibody is specific to SESN2
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: SESN2
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Dot Blot 1:10000
  • ELISA 1:10000
  • Western Blot 1:500
• Theoretical MW: 54.6 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Control Peptide:

  SESN2 Protein (NBP1-44989PEP)

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris/Glycine buffer, pH 7.4-7.8, HEPES,BSA 0.5%, glycerol 30%.
• Preservative: 0.02% Sodium Azide
• Concentration: .68 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for SESN2 Antibody

• DKFZp761M0212
• DKFZp761M02121
• Hi95
• HI95sestrin-2
• SES2
• SEST2hypoxia induced gene 95
• sestrin 2

Background

Sestrin 2(SESN2), located at human chromosome 1p35.3, belongs to the sestrin family of proteins. These proteins are cystein sulfinyl reductases and they modulate peroxide signaling and antioxidant defense (1). This family of proteins is only present in multicellular organisms ranging from nematodes to mammals. These proteins selectively reduce or repair hyperoxidized forms of typical 2-Cys peroxiredoxins within eukaryotes (2). Peroxiredoxins are the enzymes that metabolize peroxides. Expression of these proteins is regulated by p53, a tumor suppressor protein (3). Recently it was reported that sestrin 2 is not a sulfinic peroxiredoxin reductase (4). Sestrin 2 is implicated in the regulation of cell growth and survival, in cellular responses to different stress conditions, and also acts as a positive regulator of autophagy (5). Depletion of sestrin 2 in human cancer cells reduced the level of autophagy when autophagy was induced in these cells (5). Similar to sestrin 1, sestrin 2 negatively regulates the activity of mammalian target of rapamycin (mTOR), a positive regulator of cell growth (6). Sestrin 2 may be a promising target in the treatment of chronic obstructive pulmonary disease (COPD). It was reported that mutational inactivation of sestrin 2 induced TGF-Beta signaling and partially reduced the emphysema phenotype in mice model of COPD (7). Sestrin 2 might also be a key target in the treatment of cancer. It was shown to activate AMPK and repress mTORC1 signaling. AMPK is an essential mediator of LKB1 tumor suppressor and it is down-regulated in cancer cells (8). Sestrin 2 is approximately a 57.6kDa protein based on the amino acid predicted MW of 57kDa (480 amino acids).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for SESN2 Antibody (NBP1-44989)(1)

Publication using NBP1-44989

• Eid AA, Lee DY, Roman LJ et al. Sestrin 2 and AMPK connect hyperglycemia to Nox4-dependent eNOS uncoupling and matrix protein expression. Mol Cell Biol 2013-07-01 [PMID: 23816887] (WB, Rat)

Bioinformatics

• Gene Symbol: SESN2
• Entrez:
  • 83667(Human)
• Uniprot:
  • P58004(Human)