Recombinant Rhesus Macaque Serum Amyloid A1 Protein

SDS-Page: Primate Serum Amyloid A1 Protein [NBP2-35320]

• Reactivity: RM
• Applications: PAGE, Bioactivity
• Format: Carrier-Free
• Concentration: LYOPH

Recombinant Rhesus Macaque Serum Amyloid A1 Protein Summary

• Description: A single non-glycosylated polypeptide chain containing 104 amino acids corresponding to Serum Amyloid A1 Source: E. coli

  Uniprot ID: F6V9N7

  Amino Acid Sequence: RSWFSFLGEA YDGARDMWRA YSDMKEANYK NSDKYFHARG NYDAAQRGPG GVWAAEVISD ARENIQKLLG RGAEDTLADQ AANEWGRSGK DPNHFRPAGL PEKY

• Details of Functionality: Serum Amyloid A1 protein is fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human monocytes is in a concentration range of 10-100 ng/ml
• Source: E. coli
• Protein/Peptide Type: Recombinant Protein
• Gene: SAA1
• Purity: > 97 % pure by SDS-PAGE and HPLC
• Endotoxin Note: Less than 1 EU/ug of Serum Amyloid A1 as determined by LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
  • SDS-Page
• Theoretical MW: 11.7 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles.
• Buffer: Lyophilized from a 0.2 um filtered concentrated solution in PBS, pH 7.4.
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: > 97 % pure by SDS-PAGE and HPLC
• Reconstitution Instructions: Recommended to centrifuge prior to opening. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/mL.

Alternate Names for Recombinant Rhesus Macaque Serum Amyloid A1 Protein

• MGC111216
• PIG4
• SAA
• SAA1
• SAA2
• serum amyloid A protein
• Serum Amyloid A1
• TP53I4
• tumor protein p53 inducible protein 4

Background

Serum Amyloid A protein-1 (SAA1) is a multifunctional apolipoprotein that circulates as part of the HDL complex. It can displace ApoA1 as the major apolipoprotein in HDL complexes and reduce the capacity of HDL for reverse cholesterol transport. In response to proinflammatory cytokines, SAA1 is prominently produced in liver and also in human but not mouse adipose tissue. Circulating SAA1 levels can increase by as much as 1000-fold during inflammation. SAA1 induces monocyte and macrophage chemotaxis and activation through binding to CD36/SR-B3, SR-B1, FPRL1, TLR2, and RAGE. It can also opsonize and promote the clearance of Gram negative bacteria.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Bioinformatics

• Gene Symbol: SAA1