Serpin C1/Antithrombin-III Antibody

Western Blot: Serpin C1/Antithrombin-III Antibody [NBP2-76966] - Western blot analysis of SERPINC1 on different lysates using anti-SERPINC1 antibody at 1/500 dilution.Positive control:Lane1: HepG2 Lane2: Mouse smooth muscleLane3: Mouse lung

Immunocytochemistry/Immunofluorescence: Serpin C1/Antithrombin-III Antibody [NBP2-76966] - ICC staining SERPINC1 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry: Serpin C1/Antithrombin-III Antibody [NBP2-76966] - Immunohistochemical analysis of paraffin-embedded human small intestine tissue using anti-SERPINC1 antibody. Counter stained with hematoxylin.

Flow Cytometry: Serpin C1/Antithrombin-III Antibody [NBP2-76966] - Flow cytometric analysis of HepG2 cells with SERPINC1 antibody at 1/100 dilution (purple) compared with an unlabelled control (cells without incubation with primary antibody; yellow). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.

Immunocytochemistry/Immunofluorescence: Serpin C1/Antithrombin-III Antibody [NBP2-76966] - ICC staining SERPINC1 in H22 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry: Serpin C1/Antithrombin-III Antibody [NBP2-76966] - Immunohistochemical analysis of paraffin-embedded rat kidney tissue using anti-SERPINC1 antibody. Counter stained with hematoxylin.

Immunohistochemistry: Serpin C1/Antithrombin-III Antibody [NBP2-76966] - Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-SERPINC1 antibody. Counter stained with hematoxylin.

Product Discontinued

Serpin C1/Antithrombin-III Antibody Summary

• Immunogen: Recombinant protein within Human Serpin C1/Antithrombin-III aa 150-370 / 464. (SwissProt: P01008 Human; SwissProt: P32261 Mouse; SwissProt: Q5M7T5 Rat)
• Localization: Secreted.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: SERPINC1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:50-1:100
  • Immunocytochemistry/ Immunofluorescence 1:50-1:200
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Western Blot 1:500-1:1000
• Theoretical MW: 53 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH7.4), 0.2% BSA, 50% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Serpin C1/Antithrombin-III Antibody

• Antithrombin-III
• AT3antithrombin-III
• ATIII
• ATIIIantithrombin III
• MGC22579
• serine (or cysteine) proteinase inhibitor, clade C (antithrombin), member 1
• serine-cysteine proteinase inhibitor clade C member 1
• Serpin C1
• serpin peptidase inhibitor, clade C (antithrombin), member 1

Background

Antithrombin III (ATH III) is a plasma protein synthesised in the liver. Normal plasma levels are 115 to 160mg/L. ATH III consists of a single polypeptide chain and has a molecular weight of 58kDa. ATH III is a serine protease inhibitor and regulates coagulation by the formation of covalently liked complexes. ATH III also has a heparin binding site and in the presence of heparin the inhibition of thrombin, factors IXa, Xa and XIa is enhanced 1000 fold. Low levels of ATH III (functional or concentration) are associated with deep vein thrombosis and pulmonary embolism. Antithrombin assays are based on immunologic, enzymatic, or clotting properties

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Serpin C1/Antithrombin-III Antibody (NBP2-76966)(1)

Publication using NBP2-76966

• Higgins NR, Greenslade JE, Wu JJ et al. Serpin neuropathology in the P497S UBQLN2 mouse model of ALS/FTD Brain pathology (Zurich, Switzerland) 2021-03-29 [PMID: 33780087] (WB, Mouse)

Bioinformatics

• Gene Symbol: SERPINC1