Serpin C1/Antithrombin-III Antibody (2 E10) - BSA Free

Product Discontinued

Serpin C1/Antithrombin-III Antibody (2 E10) - BSA Free Summary

• Immunogen: Antithrombin III isolated from human plasma (1)
• Localization: Secreted. Found in Plasma.
• Specificity: Antithrombin III (human, native and denatured)
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: SERPINC1
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:16000
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Multiplex Immunoassay
• Application Notes: This product is useful for ELISA and Immunohistochemistry-Paraffin. This Antibody reacts strongly with AT III both native and denatured forms. A strong reaction is seen in ELISA with both native and denatured AT III directly coated onto the microtiter well and also when used as detection antibody in sandwich ELISA in combination with a polyclonal capture antibody. Use in Multiplex Immunoassay reported in scientific literature (PMID: 29898827).
• Theoretical MW: 53 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: 10mM Phosphate (pH 7.4) and 0.5M NaCl
• Preservative: 0.05% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Protein A or G purified

Alternate Names for Serpin C1/Antithrombin-III Antibody (2 E10) - BSA Free

• Antithrombin-III
• AT3antithrombin-III
• ATIII
• ATIIIantithrombin III
• MGC22579
• serine (or cysteine) proteinase inhibitor, clade C (antithrombin), member 1
• serine-cysteine proteinase inhibitor clade C member 1
• Serpin C1
• serpin peptidase inhibitor, clade C (antithrombin), member 1

Background

Antithrombin III (AT III) is a single chain glycoprotein of molecular mass 58 kDa with four glycosylation sites and three intra-molecular disulfide bonds. AT III is normally present in human plasma at a concentration of approx. 12.5 mg/dl. It is the major inhibitor of thrombin. Inactivation of thrombin by AT-III occurs by formation of a covalent bond resulting in an inactive 1:1 stoichiometric complex between the two components.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Serpin C1/Antithrombin-III Antibody (NBP1-05149)(2)

Publications using NBP1-05149

• Verbree-Willemsen L, Zhang Y, Gijsberts C et al. LDL extracellular vesicle coagulation protein levels change after initiation of statin therapy. Findings from the METEOR trial International Journal of Cardiology 2018-05-01 [PMID: 29898827] (MI, Human)
• Wang JW, Zhang YN, Sze SK, van de Weg SM. Lowering Low-Density Lipoprotein Particles in Plasma Using Dextran Sulphate Co-Precipitates Procoagulant Extracellular Vesicles. Int J Mol Sci. 2017-12-29 [PMID: 29286309] (ELISA, Human)

Bioinformatics

• Gene Symbol: SERPINC1