Mouse Serpin A7/TBG ELISA Kit (Colorimetric)

Standard Curve Reference.

Samples were spiked with high concentrations of Mouse Serpin A7/TBG, and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

• Reactivity: Mu
• Applications: ELISA
• Standard Curve Range: 0.31 - 20 ng/mL
• Sensitivity: 0.19 ng/mL

Mouse Serpin A7/TBG ELISA Kit (Colorimetric) Summary

• Description: Assay Length: 3 h 30 min
• Standard Curve Range: 0.31 - 20 ng/mL
• Sensitivity: 0.19 ng/mL
• Assay Type: Sandwich ELISA
• Inter-Assay: CV% < 10
• Intra-Assay: CV% < 10
• Spike Recovery: 80 - 120%
• Sample Volume: 100 uL
• Kit Type: ELISA Kit (Colorimetric)
• Gene: SERPINA7

Applications/Dilutions

• Dilutions:
  • ELISA

Packaging, Storage & Formulations

• Storage: Storage of components varies. See protocol for specific instructions.

Alternate Names for Mouse Serpin A7/TBG ELISA Kit (Colorimetric)

• antitrypsin), member 7
• FLJ17820
• member 7
• Serpin A7
• serpin peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin)
• T4-binding globulin
• TBG
• TBGclade A (alpha-1 antiproteinase
• thyroxin-binding globulin
• thyroxine-binding globulin

Background

Thyroxine Binding Globulin (TBG) is one of three proteins responsible for carrying the thyroid hormones thyroxine (T4) and 3,5,3 triiodothyronine (T3) in the bloodstream, along with transthyretin and albumin. TBG has the highest affinity for T4 and T3, but is present in the lowest concentration of the three proteins. Despite its low concentration, TBG carries the majority of T4 in serum. Due to the very low serum concentration of T4 & T3, TBG is rarely more than 25% saturated with ligand. Unlike transthyretin and albumin, TBG has a single binding site for T4/T3. Genomically, TBG is a serpin, although it has no inhibitory function like many other members of this class of proteins. TBG is synthesized primarily in the liver.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

FAQs for Serpin A7/TBG ELISA Kit (NBP3-49954). (Showing 1 - 1 of 1 FAQ).

1. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.

Bioinformatics

• Gene Symbol: SERPINA7