Serotonin N-acetyltransferase [p Ser206] Antibody

Product Discontinued

Serotonin N-acetyltransferase [p Ser206] Antibody Summary

• Immunogen: Synthetic peptide: C(195) H T F L R R N S(p) G C(205)
• Modification: p Ser206
• Specificity: This detects phospho serotonin N-acetyltransferase (AA-NAT) from rat tissues.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: AANAT
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:10 - 1:500
  • Western Blot 0.5 ug/ml
• Theoretical MW: 23 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Expected cross-reactivity with Mouse base on sequence homology.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS with 1 mg/ml BSA
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Serotonin N-acetyltransferase [p Ser206] Antibody

• AA-NAT
• aralkylamine N-acetyltransferaseEC 2.3.1.87
• arylalkylamine N-acetyltransferase
• Serotonin acetylase
• SNATserotonin N-acetyltransferase

Background

Pineal gland Serotonin N-acetyltransferase (Arylalkylamine N-acetyltransferase, AA-NAT) is a ~23 kDa protein which is the rate-limiting enzyme in melatonin synthesis. Melatonin serves as the hormonal signal of the daily light / dark cycle, or body clock. The nocturnal oscillation in enzyme activity ranges by 10- to 100-fold depending on species among vertebrates. Studies have shown that AA-NAT is regulated by cAMP at both the mRNA and protein levels. AA-NAT has putative protein kinase A (PKA) sites at Thr 29 and Ser 206. Phosphorylation of AA-NAT at these residues might play a role in protein inactivation via proteasome-dependent degradation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: AANAT
• Entrez:
  • 11298(Mouse)
  • 25120(Rat)
• Uniprot:
  • O88816(Mouse)
  • Q64666(Rat)