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Serine protease 23 Antibody

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Western Blot: Serine protease 23 Antibody [NBP2-93072] - Analysis of extracts of various cell lines, using Serine protease 23 . Exposure time: 90s.
Western Blot: Serine protease 23 Antibody [NBP2-93072] - Analysis of extracts of various cell lines, using Serine protease 23 . Exposure time: 90s.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

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Serine protease 23 Antibody Summary

Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 101-220 of human PRSS23 (NP_009104.2).
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
PRSS23
Purity
Affinity purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Immunohistochemistry 1:100-1:200
  • Immunohistochemistry-Paraffin
  • Western Blot 1:500-1:2000

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.3), 50% glycerol
Preservative
0.02% Sodium Azide
Purity
Affinity purified

Alternate Names for Serine protease 23 Antibody

  • EC 3.4.21
  • EC 3.4.21.-
  • EC 3.4.21.4
  • MGC5107
  • protease, serine, 23
  • Putative secreted protein Zsig13
  • serine protease 23
  • serine protease, umbilical endothelium
  • SIG13
  • SPUVE
  • ZSIG13

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Immunohistochemistry-Frozen: Fibronectin Antibody [NBP1-91258] - Feline corneal stromal cells. IHC-Fr and IF (Alexa Fluor488) were performed using NBP1-91258 at 1:400. Image captured by epifluorescent microscopy. Image from verified customer review.
Western Blot: Fibronectin Antibody [NBP1-91258] - Analysis of Fibronectin in HepG2 cell lysate.
Immunohistochemistry-Frozen: Fibronectin Antibody [NBP1-91258] - Trabecular meshwork (TM) region of pig eyes. NBP1-91258 Fibronectin antibody was labelled with Alexa Fluor 488 conjugated secondary antibody (Green). DAPI shown as blue. Image from verified customer review.
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Progesterone R/NR3C3 was detected in immersion fixed T47D human breast cancer cell line using Sheep Anti-Human Progesterone R/NR3C3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5415) at 1.7 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # NL010) and counterstained with DAPI (blue). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Progesterone R/NR3C3 was detected in immersion fixed paraffin-embedded sections of human prostate cancer tissue using Sheep Anti-Human Progesterone R/NR3C3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5415) at 5 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to nuclei. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
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Immunocytochemistry/Immunofluorescence: Carbonic Anhydrase XII/CA12 Antibody [NBP1-81668] - Staining of human cell line RT4 shows localization to nucleus. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: Carbonic Anhydrase XII/CA12 Antibody [NBP1-81668] - Staining of human kidney shows strong cytoplasmic and membranous positivity in cells in tubules.
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Western blot shows lysates of human placenta tissue. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human HTRA1/PRSS11 Monoclonal Antibody (Catalog # MAB2916) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for HTRA1/PRSS11 at approximately 51 kDa (as indicated). This experiment was conducted under non-reducing conditions and using Immunoblot Buffer Group 1.
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Immunohistochemistry-Paraffin: TFF1/pS2 Antibody [NBP1-90813] - Staining of human stomach shows high expression.
Immunohistochemistry-Paraffin: TFF1/pS2 Antibody [NBP1-90813] - Staining in human stomach and liver tissues using anti-TFF1 antibody. Corresponding TFF1 RNA-seq data are presented for the same tissues.
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Western Blot: PRSS35 Antibody [NBP2-19970] - Sample (30 ug of whole cell lysate) A: PC-3 B: SK-N-SH 10% SDS PAGE gel, diluted at 1:1000.
Western Blot: PRSS35 Antibody [NBP2-19970] - Sample (50 ug of whole cell lysate) A: Mouse Testis, 10% SDS PAGE gel, diluted at 1:1000.
Immunohistochemistry-Paraffin: PRSS35 Antibody [NBP2-19970] - Immunohistochemical analysis of paraffin-embedded U251 xenograft, using antibody at 1:500 dilution.
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Western blot shows lysates of human brain cortex tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human GPER Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5534) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for GPER at approximately 54 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
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Western Blot: NUP43 Antibody [NBP1-88792] - Analysis using Anti-NUP43 antibody NBP1-88792 (A) shows similar pattern to independent antibody NBP1-88793 (B).
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Western Blot: PSMC6 Antibody (2C4) [H00005706-M02] - Analysis of PSMC6 expression in transfected 293T cell line by PSMC6 monoclonal antibody (M02), clone 2C4.Lane 1: PSMC6 transfected lysate(44.2 KDa).Lane 2: Non-transfected lysate.
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Western Blot: PSMC6 Antibody (2C4) [H00005706-M02] - Analysis of PSMC6 over-expressed 293 cell line, cotransfected with PSMC6 Validated Chimera RNAi ( Cat # H00005706-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with PSMC6 monoclonal antibody (M02), clone 2C4 (Cat # H00005706-M02 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.
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Western Blot: RFC2 Antibody (OTI2B2) - Azide and BSA Free [NBP2-73857] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RFC2.
Flow Cytometry: RFC2 Antibody (OTI2B2) - Azide and BSA Free [NBP2-73857] - Analysis of HEK293T cells transfected with either overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostaining by RFC2 antibody.
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Immunohistochemistry-Paraffin: CDKL1 Antibody (5B11) [H00008814-M01] - Analysis of monoclonal antibody to CDKL1 on formalin-fixed paraffin-embedded human kidney. Antibody concentration 5 ug/ml.
Western Blot: CDKL1 Antibody (5B11) [H00008814-M01] - CDKL1 monoclonal antibody (M01), clone 5B11. Analysis of CDKL1 expression in human stomach.
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SGSM3 Antibody
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Immunohistochemistry-Paraffin: SGSM3 Antibody [NBP2-13304] - Staining of human stomach, lower shows strong granular cytoplasmic positivity in glandular cells.
Immunocytochemistry/Immunofluorescence: SGSM3 Antibody [NBP2-13304] - Immunofluorescent staining of human cell line MCF7 shows localization to the Golgi apparatus.
Western Blot: SGSM3 Antibody [NBP2-13304] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Human cell line RT-4
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H00003669-M01
Western Blot: ISG20 Antibody (1B2-3C9) [H00003669-M01] - Analysis of ISG20 expression in transfected 293T cell line by ISG20 monoclonal antibody (M01), clone 1B2-3C9.Lane 1: ISG20 transfected lysate(20.4 KDa).Lane 2: Non-transfected lysate.
Western Blot: ISG20 Antibody (1B2-3C9) [H00003669-M01] - BT-474, HCC1954 and SUM190 whole cell lysates were loaded with 40 ug/lane. 10% SDS-PAGE. ISG20 Antibody (H00003669-M01) was used for primary antibody: 1:1000, 4C, overnight. WB image submitted by a verified customer review.
Immunocytochemistry/Immunofluorescence: ISG20 Antibody (1B2-3C9) [H00003669-M01] - Analysis of monoclonal antibody to ISG20 on HeLa cell . Antibody concentration 10 ug/ml.
(1 Review)
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Immunohistochemistry-Paraffin: ATP6V0A1 Antibody [NBP1-89342] - Staining of human tonsil shows low expression as expected.
Immunohistochemistry-Paraffin: ATP6V0A1 Antibody [NBP1-89342] - Staining of human kidney shows strong cytoplasmic postivity in cells in tubules.
(1 Review)
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NBP1-89522
Immunocytochemistry/Immunofluorescence: DBT Antibody [NBP1-89522] - Staining of human cell line A-431 shows localization to mitochondria. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: DBT Antibody [NBP1-89522] - Staining of human pancreas shows weak to moderate cytoplasmic positivity in exocrine glandular cells.
Immunohistochemistry-Paraffin: DBT Antibody [NBP1-89522] - Staining of human testis shows weak to moderate cytoplasmic positivity in Leydig cells.
Species: Hu, Mu, Rt
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Antibody
NB600-930
Western Blot: Plasminogen Antibody [NB600-930] - Detection of Plasminogen under reducing (R) and non-reducing (NR) conditions. Reduced samples of purified target proteins contained 4% BME and were boiled for 5 minutes. Samples of 1ug of protein per lane were run by SDS-PAGE. Protein was transferred to nitrocellulose and probed with 1:3000 dilution of primary antibody. Detection shown was using Dylight 649 conjugated Donkey anti goat 1 hr RT.
Western Blot: Plasminogen Antibody [NB600-930] - Lane 1: Plasminogen. Lane 2: None. Load: 50 ng per lane. Primary antibody: Plasminogen primary antibody at 1:1,000 overnight at 4C. Secondary antibody: Peroxidase goat secondary antibody at 1:40,000 for 60 min at RT. Blocking: incubated with blocking buffer for 30 min at RT. Predicted/Observed size: 91 kDa, 91 kDa for Plasminogen. Other band(s): None.
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Immunocytochemistry/Immunofluorescence: RFC4 Antibody (OTI1A8) [NBP2-45946] - Analysis of COS7 cells transiently transfected by pCMV6-ENTRY RFC4.
Immunohistochemistry: RFC4 Antibody (OTI1A8) [NBP2-45946] - Analysis of Carcinoma of Human pancreas tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
Immunohistochemistry: RFC4 Antibody (OTI1A8) [NBP2-45946] - Analysis of Human tonsil tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
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NBP1-83224
Immunocytochemistry/Immunofluorescence: Cbx8 Antibody [NBP1-83224] - Staining of human cell line A-431 shows localization to nucleoplasm. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: Cbx8 Antibody [NBP1-83224] - Staining of human liver shows no positivity in hepatocytes as expected.
Immunohistochemistry-Paraffin: Cbx8 Antibody [NBP1-83224] - Staining of human colon shows moderate to strong cytoplasmic positivity in glandular cells.
Species: Hu
Applications: ICC/IF, IHC, IHC-P, WB
ACTR1B Antibody
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Western Blot: ACTR1B Antibody [NBP2-15275] - Sample (50 ug of whole cell lysate) A: mouse brain 10% SDS PAGE; antibody diluted at 1:1000.
Western Blot: ACTR1B Antibody [NBP2-15275] - Sample (30 ug of whole cell lysate) A: IMR32 10% SDS PAGE; antibody diluted at 1:1000.
Immunohistochemistry-Paraffin: ACTR1B Antibody [NBP2-15275] - Paraffin-embedded ovarian carcinoma. ACTR1B antibody dilution: 1:500.
Species: Hu, Mu
Applications: ICC/IF, IHC, IHC-P, WB

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Bioinformatics

Gene Symbol PRSS23