Semaphorin 7A Antibody

Western Blot: Semaphorin 7A Antibody [NBP1-32699] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with SEMA7A antibody [C1C3] diluted at 1:1000.

Immunohistochemistry-Paraffin: Semaphorin 7A Antibody [NBP1-32699] - Cal27 xenograft , using CD108 antibody at 1:100 dilution. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.

Western Blot: Semaphorin 7A Antibody [NBP1-32699] - Various tissue extracts (50 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with SEMA7A antibody [C1C3] diluted at 1:1000.

Product Discontinued

Semaphorin 7A Antibody Summary

• Immunogen: Recombinant protein encompassing a sequence within the center region of human SEMA7A. The exact sequence is proprietary.
• Localization: Cell membrane, Lipid-anchor, GPI-anchor
• Predicted Species: Rhesus Macaque (97%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: SEMA7A
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:500-1:3000
• Application Notes: IHC-Fr (Frozen sections) Assay dependent .
• Theoretical MW: 75 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Bovine (81%).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: 0.1M Tris (pH 7), 0.1M Glycine, 10% Glycerol
• Preservative: 0.01% Thimerosal
• Purity: Immunogen affinity purified

Alternate Names for Semaphorin 7A Antibody

• CD108 antigen
• CD108
• CD108MGC126696
• CDw108
• H-SEMA-K1
• H-Sema-L
• JMH blood group antigen
• JMH
• John-Milton-Hargen human blood group Ag
• MGC126692
• sema domain, immunoglobulin domain (Ig), and GPI membrane anchor, (semaphorin)7A (JMH blood group)
• sema domain, immunoglobulin domain (Ig), and GPI membrane anchor, (semaphorin)7A
• sema domain, immunoglobulin domain (Ig), and GPI membrane anchor, 7A
• Sema K1
• Sema L
• Sema7A
• SEMAK1
• SEMAL
• SEMALJohn Milton Hagen blood group H-Sema K1
• Semaphorin 7A
• semaphorin 7A, GPI membrane anchor (John Milton Hagen blood group)
• semaphorin K1
• semaphorin L
• semaphorin-7A
• semaphorin-K1
• semaphorin-L

Background

SEMA7A is a membrane-bound semaphorin that associates with cell surfaces via a glycosylphosphatidylinositol (GPI) linkage. SEMA7A is also known as the John-Milton-Hagen (JMH) blood group antigen, an 80-kD glycoprotein expressed on activated lymphocytes and erythrocytes.[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Publications for Semaphorin 7A Antibody (NBP1-32699)(1)

Publication using NBP1-32699

• Manini I, Ruaro ME, Sgarra R et al. Semaphorin-7A on Exosomes: A Promigratory Signal in the Glioma Microenvironment Cancers (Basel) 2019-05-30 [PMID: 31151295] (WB, Human)

Bioinformatics

• Gene Symbol: SEMA7A
• Entrez:
  • 8482(Human)
• Uniprot:
  • O75326()