Semaphorin 4B Antibody

Western blot shows lysates of mouse pancreas tissue. PVDF Membrane was probed with 1 µg/mL of Sheep Anti-Mouse Semaphorin 4B Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6239) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Semaphorin 4B at approximately 100 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

• Reactivity: Mu
• Applications: WB
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Unconjugated
• Concentration: LYOPH

Semaphorin 4B Antibody Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant mouse Semaphorin 4B
  Leu31-Glu703
  Accession # Q62179
• Specificity: Detects mouse Semaphorin 4B in direct ELISAs and Western blots. In Western blots, approximately 25% cross-reactivity with recombinant human Semaphorin 4B is observed and less than 2% cross-reactivity with recombinant mouse (rm) Semaphorin 4A and rmSemaphorin 4C is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Gene: SEMA4B
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Semaphorin 4B Antibody

• KIAA1745sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and shortcytoplasmic domain, 4B
• MGC131831
• sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and shortcytoplasmic domain, (semaphorin) 4B
• SEMA4B
• SEMAC
• Semaphorin 4B
• semaphorin-4B
• SemC

Background

Semaphorin 4B (Sema4B; also SemaC) is a 95-100 kDa, class IV member of the semaphorin family of proteins. It is expressed in neurons, and following PSD-95 induced clustering, participates in the formation or functioning of glutamatergic synapses. Mature mouse Sema4B is a type I transmembrane glycoprotein that is 793 amino acids (aa) in length. It contains a 673 aa extracellular region (aa 31-703) that is characterized by one Sema domain (aa 57-492), a PSI region (aa 512-582), and an Ig-like C2-type domain (aa 589-649). There are three phosphoserines in the cytoplasmic tail. Over aa 31-703, mouse Sema4B shares 86% and 95% aa identity with human and rat Sema4B, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: SEMA4B
• Uniprot:
  • Q62179()