Semaphorin 3E Antibody

Immunohistochemistry: Semaphorin 3E Antibody [NBP1-84408] - Staining of mouse midbrain shows strong immunoreactivity in the dorsal raphe neurons.

Immunohistochemistry-Paraffin: Semaphorin 3E Antibody [NBP1-84408] - Staining of human duodenum shows strong cytoplasmic positivity in glandular cells.

Immunohistochemistry-Paraffin: Semaphorin 3E Antibody [NBP1-84408] - Staining of human cerebral cortex shows staining in blood vessels and moderate positivity in neurons.

Immunohistochemistry-Paraffin: Semaphorin 3E Antibody [NBP1-84408] - Staining of human hippocampus shows positivity in blood vessels and neurons.

Immunohistochemistry-Paraffin: Semaphorin 3E Antibody [NBP1-84408] - Staining of human lateral ventricle shows strong cytoplasmic positivity in neurons.

Immunohistochemistry: Semaphorin 3E Antibody [NBP1-84408] - Staining of mouse hypothalamus shows postivity in a subset of neurons in the anterior hypothalamic area.

Immunohistochemistry: Semaphorin 3E Antibody [NBP1-84408] - Staining of mouse caudate putamen shows cytoplasmic immunoreactivity in neurons.

Immunohistochemistry: Semaphorin 3E Antibody [NBP1-84408] - Staining of mouse hippocampus shows cytoplasmic positivity in the CA2 pyramidal neurons.

Immunohistochemistry: Semaphorin 3E Antibody [NBP1-84408] - Staining of mouse visual cortex shows cytoplasmic immunoreactivity in neurons.

Product Discontinued

Semaphorin 3E Antibody Summary

• Immunogen: This antibody was developed against Recombinant Protein corresponding to amino acids: MDLGLLFLRLHKSDAGTYFCQTVEHSFVHTVRKITLEVVEEEKVEDMFNKDDEEDRHHRMPCPAQSSISQGAKPWYKEFLQLIGYSNFQRVEEYCEKVWCTDRKRKKLKMSPSKWKYANPQEKKLRSKPEHYR
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: SEMA3E
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:200 - 1:500
  • Immunohistochemistry-Paraffin 1:200 - 1:500
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Rat (86%)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Immunogen affinity purified

Alternate Names for Semaphorin 3E Antibody

• (semaphorin) 3E
• coll-5
• KIAA0331M-SEMAH
• sema domain, immunoglobulin domain (Ig), short basic domain, secreted
• Sema3E
• SEMAH
• SEMAHM-SemaK
• Semaphorin 3E
• semaphorin-3E

Background

Semaphorins, such as Semaphorin 3E, are characterized by a conserved domain of about 500 amino acids. These proteins are involved in embryonic development, and some behave as neural guidance molecules.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: SEMA3E