Semaphorin 3A Antibody (215803) [Phycoerythrin]

U-118 MG cells were stained with Mouse Anti-Human/Mouse Semaphorin 3A PE‑conjugated Monoclonal Antibody (Catalog # IC1250P, filled histogram) or isotype control antibody (Catalog # IC0041P, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

CD3+-gated human peripheral blood mononuclear cells (PBMCs) treated with PHA were stained with Mouse Anti-Human/Mouse Semaphorin 3A PE-conjugated Monoclonal Antibody (Catalog # IC1250P, filled histogram) or isotype control antibody (Catalog # IC0041P, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

Mouse splenocytes treated with PHA were stained with Rat Anti-Mouse CD3 APC-conjugated Monoclonal Antibody (Catalog # FAB4841A) and either (A) Mouse Anti-Human/Mouse Semaphorin 3A PE-conjugated Monoclonal Antibody (Catalog # IC1250P) or (B) Mouse IgG2BPhycoerythrin Isotype Control (Catalog # IC0041P). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

• Reactivity: Hu, Mu
• Applications: Flow
• Clone: 215803
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Phycoerythrin

Semaphorin 3A Antibody (215803) [Phycoerythrin] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human Semaphorin 3A
  Lys26-Val771 (Arg555Ala, Arg552Ala)
  Accession # Q14563
• Specificity: Detects human Semaphorin 3A in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human Semaphorin 3B, 3E, 6A, 6B, 6C, 6D, 7A, recombinant mouse Semaphorin 3C, or 3F is observed.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: SEMA3A
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Intracellular Staining by Flow Cytometry 10 uL/10^6 cells

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze.
  12 months from date of receipt, 2 to 8 degreesC as supplied.
• Buffer: Supplied in a saline solution containing BSA and Sodium Azide.
• Preservative: Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Semaphorin 3A Antibody (215803) [Phycoerythrin]

• (semaphorin) 3A
• coll-1
• collapsin 1
• Hsema-I
• Hsema-III
• MGC133243
• sema domain, immunoglobulin domain (Ig), short basic domain, secreted
• Sema III
• SEMA1
• Sema3A
• SEMAD
• SEMAIII
• SEMAL
• Semaphorin 3A
• semaphorin D
• Semaphorin III
• semaphorin L
• semaphorin-3A
• SemD

Background

The Semaphorins constitute a large family of secreted, GPI-anchored and transmembrane cell signaling molecules. Depending on their domain organization and species origin, these proteins can be classified into eight groups. To date, at least 19 vertebrate Semaphorins belonging to five groups (class 3 through 7) have been identified. All Semaphorins contain a conserved, 500 amino acid (aa) Sema domain at the amino terminus. Semaphorins are best known for their roles in axon guidance during neuronal development. Semaphorins are also expressed in non-neuronal tissues and are involved in angiogenesis, hematopoiesis, organogenesis, and the regulation of immune functions (1, 2). Class 3 Semaphorins (Sema3) are secreted proteins containing a Sema domain, an immunoglobulin c2-like domain and a basic domain near the carboxyl tail. Sema3A (also referred to as SemaIII, SemD and Collapsin) cDNA predicts a 771 aa precursor protein with a putative 25 aa signal peptide (1-3). Bioactive Sema3A is a disulfide-linked dimer (4). The bioactivity is increased after proteolytic processing by a furin-like endoprotease near the carboxy-terminus (1). The functional receptor complex for Sema3 is composed of two distinct transmembrane proteins: Neuropilin-1 (Npn-1) and Plexin-A. Npn-1 binds directly to Sema3A with high-affinity and confers specificity. Plexin-A interacts with Npn-1 to increase the affinity of the complex for Sema3A and serves as the signaling subunit in the receptor complex (1, 2, 5).

1. Nakamura, F. et al. (2000) J Neurobiol. 44:219.
2. Goshima, Y. et al. (2002) J. Clin. Invest. 109:993.
3. Kolodkin, A.L. et al. (1993) Cell 75:1389.
4. Koppel, A.M. et al. (1998) J. Biol. Chem. 273:15708.
5. Yu, T.W. et al. (2001) Nature Neurosci. Supplement 4:1169.
6. Luo Y. et al. (1993) Cell 75:217.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Semaphorin 3A Antibody (IC1250P)(1)

Publication using IC1250P

• Barnkob, MB;Michaels, YS;André, V;Macklin, PS;Gileadi, U;Valvo, S;Rei, M;Kulicke, C;Chen, JL;Jain, V;Woodcock, VK;Colin-York, H;Hadjinicolaou, AV;Kong, Y;Mayya, V;Mazet, JM;Mead, GJ;Bull, JA;Rijal, P;Pugh, CW;Townsend, AR;Gérard, A;Olsen, LR;Fritzsche, M;Fulga, TA;Dustin, ML;Jones, EY;Cerundolo, V; Semmaphorin 3?A causes immune suppression by inducing cytoskeletal paralysis in tumour-specific CD8+ T cells Nature communications 2024-04-12 [PMID: 38609390] (Flow Cytometry, Mouse)

Bioinformatics

• Gene Symbol: SEMA3A
• Uniprot:
  • Q14563()