Semaphorin 3A Antibody (215803) [Phycoerythrin]

Images

 
CD3+-gated human peripheral blood mononuclear cells (PBMCs) treated with PHA were stained with Mouse Anti-Human/Mouse Semaphorin 3A PE‑conjugated Monoclonal Antibody (Catalog # IC1250P, filled histogram) or isotype ...read more
Mouse splenocytes treated with PHA were stained with Rat Anti-Mouse CD3 APC‑conjugated Monoclonal Antibody (Catalog # FAB4841A) and either (A) Mouse Anti-Human/Mouse Semaphorin 3A PE‑conjugated Monoclonal Antibody ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications Flow
Clone
215803
Clonality
Monoclonal
Host
Mouse
Conjugate
Phycoerythrin

Semaphorin 3A Antibody (215803) [Phycoerythrin] Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant human Semaphorin 3A
Lys26-Val771 (Arg555Ala, Arg552Ala)
Accession # Q14563
Specificity
Detects human Semaphorin 3A in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross‑reactivity with recombinant human Semaphorin 3B, 3E, 6A, 6B, 6C, 6D, 7A, recombinant mouse Semaphorin 3C, or 3F is observed.
Source
N/A
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Gene
SEMA3A
Purity
Protein A or G purified from hybridoma culture supernatant
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Intracellular Staining by Flow Cytometry 10 uL/10^6 cells

Packaging, Storage & Formulations

Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.
Buffer
Supplied in a saline solution containing BSA and Sodium Azide.
Preservative
Sodium Azide
Purity
Protein A or G purified from hybridoma culture supernatant

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Semaphorin 3A Antibody (215803) [Phycoerythrin]

  • (semaphorin) 3A
  • coll-1
  • collapsin 1
  • Hsema-I
  • Hsema-III
  • MGC133243
  • sema domain, immunoglobulin domain (Ig), short basic domain, secreted
  • Sema III
  • SEMA1
  • Sema3A
  • SEMAD
  • SEMAIII
  • SEMAL
  • Semaphorin 3A
  • semaphorin D
  • Semaphorin III
  • semaphorin L
  • semaphorin-3A
  • SemD

Background

The Semaphorins constitute a large family of secreted, GPI-anchored and transmembrane cell signaling molecules. Depending on their domain organization and species origin, these proteins can be classified into eight groups. To date, at least 19 vertebrate Semaphorins belonging to five groups (class 3 through 7) have been identified. All Semaphorins contain a conserved, 500 amino acid (aa) Sema domain at the amino terminus. Semaphorins are best known for their roles in axon guidance during neuronal development. Semaphorins are also expressed in non-neuronal tissues and are involved in angiogenesis, hematopoiesis, organogenesis, and the regulation of immune functions (1, 2).

Class 3 Semaphorins (Sema3) are secreted proteins containing a Sema domain, an immunoglobulin c2-like domain and a basic domain near the carboxyl tail. Sema3A (also referred to as SemaIII, SemD and Collapsin) cDNA predicts a 771 aa precursor protein with a putative 25 aa signal peptide (1‑3). Bioactive Sema3A is a disulfide-linked dimer (4). The bioactivity is increased after proteolytic processing by a furin-like endoprotease near the carboxy-terminus (1). The functional receptor complex for Sema3 is composed of two distinct transmembrane proteins: Neuropilin-1 (Npn-1) and Plexin-A. Npn-1 binds directly to Sema3A with high-affinity and confers specificity. Plexin-A interacts with Npn-1 to increase the affinity of the complex for Sema3A and serves as the signaling subunit in the receptor complex (1, 2, 5).

  1. Nakamura, F. et al. (2000) J Neurobiol. 44:219.
  2. Goshima, Y. et al. (2002) J. Clin. Invest. 109:993.
  3. Kolodkin, A.L. et al. (1993) Cell 75:1389.
  4. Koppel, A.M. et al. (1998) J. Biol. Chem. 273:15708.
  5. Yu, T.W. et al. (2001) Nature Neurosci. Supplement 4:1169.
  6. Luo Y. et al. (1993) Cell 75:217.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol SEMA3A
Uniprot