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MAB0041, open histogram) followed by Fluorescein-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0103B). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules." class="big_lightbox" target="_blank">
AB-108-C, open histogram) followed by Phycoerythrin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0107). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules." class="big_lightbox" target="_blank">
566-N1) is coated at 2 µg/ml, Recombinant Human Semaphorin 3A Fc Chimera (Catalog # 1250-S3) binds with an ED50 = 50-300 ng/mL." class="big_lightbox" target="_blank">
![Intracellular Staining by Flow Cytometry Semaphorin 3A Antibody (215803) [Unconjugated]](http://images.novusbio.com/images/antibody/mab1250_human-semaphorin-3a-mab-clone-215803-intracellular-staining-by-flow-cytometry-662024141032..jpg)
![Flow Cytometry Semaphorin 3A Antibody [Unconjugated]](http://images.novusbio.com/images/antibody/af1250_human-semaphorin-3a-affinity-purified-polyclonal-ab-flow-cytometry-1992025131228.jpg)
![N/A Semaphorin 3A [Biotin]](http://images.novusbio.com/fullsize/dy1250-05_human-semaphorin-3a-duoset-elisa-5-plate-2332022122452.jpg)
![Bioactivity Semaphorin 3A [Unconjugated]](http://images.novusbio.com/images/protein/Semaphorin_3A_1250S3_1473.jpg)
![Western Blot Semaphorin 3A Antibody (215803) [Unconjugated]](http://images.novusbio.com/fullsize/mab1250_human-semaphorin-3a-mab-clone-215803-western-blot-121220257455223.jpg)
![Western Blot Semaphorin 3A Antibody (215803) [Unconjugated]](http://images.novusbio.com/fullsize/mab1250_human-semaphorin-3a-mab-clone-215803-western-blot-12122025931330.jpg)
