Seipin/BSCL2 Antibody [Alexa Fluor® 350]

• Reactivity: Hu
• Applications: WB, ICC/IF
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Alexa Fluor 350

Seipin/BSCL2 Antibody [Alexa Fluor® 350] Summary

• Immunogen: E. coli-derived recombinant human Seipin/BSCL2
  Asn279-Ser398
  Accession # Q96G97
• Specificity: Detects human Seipin/BSCL2 in direct ELISAs and Western blots.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry
  • Western Blot

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Seipin/BSCL2 Antibody [Alexa Fluor® 350]

• Berardinelli-Seip congenital lipodystrophy 2 (seipin)
• Bernardinelli-Seip congenital lipodystrophy 2 (seipin)
• Bernardinelli-Seip congenital lipodystrophy type 2 protein
• BSCL2
• FLJ16651
• GNG3LG
• HMN5
• MGC4694
• Seipin
• spastic paraplegia 17 (Silver syndrome)
• SPG17

Background

BSCL2 (Bernardinelli-Seip Congenital Lipodystrophy type 2 protein; also Seipin) is a 70-74 kDa glycoprotein member of the seipin family of molecules. Although it has widespread expression, and has been specifically found in the endoplasmic reticulum (ER) of cortical and spinal motor neurons, basophils of the anterior pituitary, adipocytes and spermatids. Its function is unclear, but it is known to structurally resemble SREBPs, and thus may regulate gene transcription. Mutations are reported that involve its glycosylation site, causing protein misfolding and the initiation of apoptosis. Human BSCL2 is 398 amino acids (aa) in length. It contains two transmembrane segments (aa 27-47 and 243-263) plus an N-terminal (aa 1-26) and C-terminal (aa 264-398) cytoplasmic domain. BSCL2 is polyubiquitinated, and runs between 100-200 kDa in SDS-PAGE. There is an alternative start site 64 aa upstream of the standard site. This 462 aa isoform is considered to be the predominant isoform for BSCL2, and is associated with the 70-74 kDa MW cited above. There is one additional potential isoform variant that possesses a 63 aa substitution for aa 225-398. BSCL2 is reportedly cleaved, and generates a 40-41 kDa N-terminal fragment. Over aa 279-398, human BSCL2 shares 73% aa sequence identity with mouse BSCL2.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.