SEC13 Antibody [Unconjugated]

Western blot shows lysates of human kidney tissue and human lung tissue. PVDF membrane was probed with 0.05 µg/mL of Rabbit Anti-Human SEC13 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF9055) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for SEC13 at approximately 37 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

SEC13 was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Rabbit Anti-Human SEC13 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF9055) at 1.7 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rabbit IgG Secondary Antibody (red; Catalog # NL004) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm and nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Simple Western lane view shows lysates of K562 human chronic myelogenous leukemia cell line, loaded at 0.2 mg/mL. A specific band was detected for SEC13 at approximately 42 kDa (as indicated) using 0.5 µg/mL of Rabbit Anti-Human SEC13 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF9055). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

• Reactivity: Hu
• Applications: WB, Simple Western, ICC/IF
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

SEC13 Antibody [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant human SEC13
  Gly123-Gln322
  Accession # P55735
• Specificity: Detects human SEC13 in direct ELISA and Western blots.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry 1-15 ug/mL
  • Simple Western 0.5 ug/mL
  • Western Blot 0.05 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for SEC13 Antibody [Unconjugated]

• D3S1231E
• D3S1231ESEC13L1npp-20
• Npp-20
• protein SEC13 homolog
• SEC13 (S. cerevisiae)-like 1
• SEC13 homolog (S. cerevisiae)
• SEC13
• SEC13L1
• SEC13-like 1 isoform
• SEC13-like protein 1
• SEC13R
• SEC13-related protein
• SEC13RSEC13-like 1 (S. cerevisiae)

Background

Sec13 is a ubiquitously expressed protein which participates in the formation of vesicles in the COPII complex with Sec23p^.Sec24p, Sar1p and Sec31. The COPII vesicle budding complex was first described in the yeast system and was shown to provide coat proteins in the early secretory pathway. Sec13 is recruited to endoplasmic reticulum (ER) membranes where it forms a three dimensional cuboctahedron cage lattice structure by association with Sec31. This vesicular structure is necessary for ER-Golgi transport.  Presenillin-1, which is implicated in early-onset Alzheimer's disease has been shown to interact with Sec13 vesicles.  Additionally, Sec13 has been shown to be a component of the Y-shaped Nup107-160 subcomplex of the nuclear pore complex (NPC), and the GATOR2 sub-complex of the GATOR complex.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for SEC13 Antibody (AF9055)(3)

Publications using AF9055

• Nalbach K, Schifferer M, Bhattacharya D et al. Spatial proteomics reveals secretory pathway disturbances caused by neuropathy-associated TECPR2 Nature communications 2/16/2023 [PMID: 36797266]
• Franziska Eck, Santosh Phuyal, Matthew D. Smith, Manuel Kaulich, Simon Wilkinson, Hesso Farhan et al. ACSL3 is a novel GABARAPL2 interactor that links ufmylation and lipid droplet biogenesis Journal of Cell Science 1/1/2020 [PMID: 32843575]
• Nalbach K, Schifferer M, Lichtenthaler S et al. Spatial Proteomics Reveals Disturbances in Trafficking and Interactions Along the Secretory Pathway Upon Loss of Neuropathy-Associated TECPR2 SSRN Electronic Journal Mar 16 2022 (ICC/IF, WB, Human)

Bioinformatics

• Uniprot:
  • P55735()