SDHB Antibody (SDHB/6697R)

Immunohistochemistry-Paraffin: SDHB Antibody (SDHB/6697R) [NBP3-07818] - Formalin-fixed, paraffin-embedded human heart stained with SDHB Recombinant Rabbit Monoclonal Antibody (SDHB/6697R).

Immunohistochemistry-Paraffin: SDHB Antibody (SDHB/6697R) [NBP3-07818] - Formalin-fixed, paraffin-embedded human paraganglioma stained with SDHB Recombinant Rabbit Monoclonal Antibody (SDHB/6697R).

• Reactivity: Hu
• Applications: IHC
• Clone: SDHB/6697R
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 0.2 mg/ml

SDHB Antibody (SDHB/6697R) Summary

• Description: 200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP3-08827)
  
  Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.
• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: Synthetic peptide corresponding to residues in human SDHB was used as an immunogen. (Uniprot: P21912 )
• Localization: Cytoplasm, Mitochondria
• Marker: Pheochromocytoma Marker
• Isotype: IgG Kappa
• Clonality: Monoclonal
• Host: Rabbit
• Gene: SDHB
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry-Paraffin 1-2 ug/ml
• Application Notes: Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
  Optimal dilution for a specific application should be determined.
• Theoretical MW: 32 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C.
• Buffer: 10 mM PBS with 0.05% BSA
• Preservative: 0.05% Sodium Azide
• Concentration: 0.2 mg/ml
• Purity: Protein A or G purified

Alternate Names for SDHB Antibody (SDHB/6697R)

• EC 1.3.5.1
• FLJ92337
• IP
• Iron-sulfur subunit of complex II
• PGL4
• SDH
• SDH1
• SDH2
• SDHIP
• succinate dehydrogenase [ubiquinone] iron-sulfur subunit, mitochondrial
• succinate dehydrogenase complex, subunit B, iron sulfur (Ip)

Background

Complex II of the respiratory chain, which is specifically involved in the oxidation of succinate, carries electrons from FADH to CoQ. The complex is composed of four nuclear-encoded subunits and is localized in the mitochondrial inner membrane. The iron-sulfur subunit is highly conserved and contains three cysteine-rich clusters which may comprise the iron-sulfur centers of the enzyme. Sporadic and familial mutations in this gene result in paragangliomas and pheochromocytoma, and support a link between mitochondrial dysfunction and tumorigenesis. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: SDHB
• Uniprot:
  • P21912(Human)