SCL/Tal1 Antibody (C.15200012)

Immunocytochemistry/Immunofluorescence: SCL/Tal1 Antibody (C.15200012) [NBP2-59207] - K562 cells were stained with the antibody against TAL1 and with DAPI. Cells were fixed with 4% formaldehyde for 10 minutes and blocked with PBS/Triton X-100 containing 1% BSA. The cells were immunofluorescently labelled with the TAL1 antibody (middle) diluted 1:1000 in blocking solution followed by an anti-mouse antibody conjugated to Alexa Fluor 594. The left panel shows staining of the nuclei with DAPI. A merge of both stainings is shown on the right.

Chromatin Immunoprecipitation: SCL/Tal1 Antibody (C.15200012) [NBP2-59207] - ChIP was performed using K562 cells, the antibody against TAL1 and optimized PCR primer sets for qPCR. ChIP was performed with a ChIP-seq kit, using sheared chromatin from 4 million cells. A titration of the antibody consisting of 1, 2, 5 and 10 ug per ChIP experiment was analysed. IgG (2 ug/IP) was used as negative IP control. Quantitative PCR was performed with primers for the RUNX1 and RUNX1T1 genes, used as positive controls, and for the coding region of the inactive MYOD1 gene and the Sat2 satellite repeat, used as negative controls. Figure shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).

Product Discontinued

SCL/Tal1 Antibody (C.15200012) Summary

• Immunogen: The exact immunogen is propietary information.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: TAL1
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Chromatin Immunoprecipitation (ChIP) 2-5 ug/IP
  • Chromatin Immunoprecipitation Sequencing 2 - 5ug/ChIP
  • Immunocytochemistry/ Immunofluorescence 1:1000

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.05% Sodium Azide
• Purity: Affinity purified

Alternate Names for SCL/Tal1 Antibody (C.15200012)

• BHLHA17
• bHLHa17tal-1
• Class A basic helix-loop-helix protein 17
• SCL
• SCLT-cell acute lymphocytic leukemia protein 1
• Stem Cell Protein
• Tal1
• TAL-1
• T-cell acute lymphocytic leukemia 1
• T-cell leukemia/lymphoma protein 5
• TCL5
• TCL5tal-1 product

Background

TAL1 (UniProt/Swiss-Prot entry P17542) is a transcription factor involved in hemopoietic differentiation It functions as a positive regulator of erythroid differentiation. TAL1 is implicated in the genesis of hemopoietic malignancies such as T-cell leukemia and cd3epsilon deficiency.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: TAL1