SCIN Antibody - BSA Free

Western Blot: SCIN Antibody [NBP1-31721] - Sample (30 ug of whole cell lysate) A: Hep G2 7. 5% SDS PAGE; antibody diluted at 1:1000.

Immunocytochemistry/Immunofluorescence: SCIN Antibody [NBP1-31721] - Analysis of methanol-fixed HeLa, using SCIN antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with an alpha Tubulin antibody (Red) at 1:2000.

• Reactivity: Hu, Mu, Rt, Po
• Applications: WB, ICC/IF
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

SCIN Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit SCIN Antibody - BSA Free (NBP1-31721) is a polyclonal antibody validated for use in WB and ICC/IF. Anti-SCIN Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Recombinant protein encompassing a sequence within the center region of human SCIN. The exact sequence is proprietary.
• Localization: Cytoplasm, cytoskeleton
• Predicted Species: Mouse (92%), Rat (92%), Porcine (94%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: SCIN
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Western Blot 1:500-1:3000
• Theoretical MW: 80 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Chicken (83%), Bovine (87%).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: 0.1M Tris, 0.1M Glycine, 10% Glycerol
• Preservative: 0.01% Thimerosal
• Purity: Antigen Affinity-purified

Alternate Names for SCIN Antibody - BSA Free

• KIAA1905adseverin
• Scinderin

Background

Ca(2+)-dependent actin filament-severing protein that is presumed to have a regulatory function in exocytosisby affecting the organization of the microfilament network underneath the plasma membrane. In vitro, also has barbedend capping and nucleating activities in the presence of Ca(2+)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Publications for SCIN Antibody (NBP1-31721)(1)

Publication using NBP1-31721

• Matsumura T, Totani H, Gunji Y et al. A Myb enhancer-guided analysis of basophil and mast cell differentiation Nature communications 2022-11-18 [PMID: 36400777] (ICC/IF, Mouse)

Bioinformatics

• Gene Symbol: SCIN