SCF/c-kit Ligand Antibody

Recombinant Mouse SCF/c‑kit Ligand (Catalog # 455‑MC) stimulates proliferation in the TF‑1 human erythro­leukemic cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Mouse SCF/c‑kit Ligand (25 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Mouse SCF/c-kit Ligand Polyclonal Antibody (Catalog # AB-455-NA). The ND50 is typically 4-20 µg/mL.

• Reactivity: Mu
• Applications: WB, B/N
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

SCF/c-kit Ligand Antibody Summary

• Immunogen: E. coli-derived recombinant mouse SCF/c‑kit Ligand
  Lys26-Ala189
  Accession # Q78ED8
• Specificity: Detects mouse SCF/c‑kit Ligand in direct ELISAs and Western blots. In direct ELISAs, approximately 70% cross-reactivity with recombinant rat SCF/c-kit Ligand is observed, and approximately 25% cross-reactivity with recombinant human SCF/c‑kit Ligand and recombinant feline SCF/c‑kit Ligand is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: Kitl
• Purity Statement: Protein A or G purified
• Endotoxin Note: <0.10 EU per 1 μg of the antibody by the LAL method.

Applications/Dilutions

• Dilutions:
  • Neutralization 4-20 ug/mL
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 1 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for SCF/c-kit Ligand Antibody

• c-kit Ligand
• DCUA
• DFNA69
• DKFZp686F2250
• familial progressive hyperpigmentation 2
• FPH2
• FPHH
• KIT ligand
• Kitl
• KITLG
• KL-1
• Mast cell growth factor
• MGF
• MGFSHEP7
• SCF
• SCFStem cell factor
• SFc-Kit ligand
• SHEP7
• SLF
• steel factor

Background

Stem cell factor (SCF), also known as c-kit ligand (KL), mast cell growth factor (MGF), and steel factor (SLF), is a widely expressed 28-40 kDa type I transmembrane glycoprotein (1). It promotes the survival, differentiation, and mobilization of multiple cell types including myeloid, erythroid, megakaryocytic, lymphoid, germ cell, and melanocyte progenitors (1-7). SCF is a primary growth and activation factor for mast cells and eosinophils (8). Mature mouse SCF consists of a 189 amino acid (aa) extracellular domain (ECD), a 23 aa transmembrane segment, and a 36 aa cytoplasmic tail (9). The ECD shows both N-linked and O-linked glycosylation (10). Proteolytic cleavage at two alternate sites in the extracellular juxtamembrane region releases a 25 kDa soluble molecule which is comparable to the only form produced by Steel-dickie mutant mice (11, 12). An alternately spliced isoform of mouse SCF lacks 28 aa that encompasses the primary proteolytic recognition site (13). Within the ECD of the short isoform (corresponding to this recombinant protein), mouse SCF shares 93% aa sequence identity with rat SCF and 72-75% with canine, feline, and human SCF. Rat SCF is active on mouse and human cells, but human SCF is only weakly active on mouse cells (14). Non-covalent dimers of transmembrane or soluble SCF interact with the receptor tyrosine kinase SCF R/c-kit to trigger receptor dimerization and signaling (15). SCF assists in the recovery of cardiac function following myocardial infarction by increasing the number of cardiomyocytes and vascular channels (16).

1. Ashman, L.K. (1999) Int. J. Biochem. Cell Biol. 31:1037.
2. Sette, C. et al. (2000) Int. J. Dev. Biol. 44:599. 
3. Yoshida, H. et al. (2001) J. Invest. Dermatol. Symp. Proc. 6:1.
4. Erlandsson, A. et al. (2004) Exp. Cell Res. 301:201. 
5. Kapur, R. et al. (2002) Blood 100:1287.
6. Wang, C.-H. et al. (2007) Arterioscler. Thromb. Vasc. Biol. 27:540.
7. Bashamboo, A. et al. (2006) J. Cell Sci. 119:3039. 
8. Reber, L. et al. (2006) Eur. J. Pharmacol. 533:327. 
9. Huang, E. et al. (1990) Cell 63:225.
10. Arakawa, T. et al. (1991) J. Biol. Chem. 266:18942.
11. Majumdar, M.K. et al. (1994) J. Biol. Chem. 269:1237.
12. Brannan, C.I. et al. (1991) Proc. Natl. Acad. Sci. USA 88:4671.
13. Flanagan, J.G. et al. (1991) Cell 64:1025.
14. Martin, F.H. et al. (1990) Cell 63:203.
15. Lemmon, M.A. et al. (1997) J. Biol. Chem. 272:6311.
16. Kanellakis, P. et al. (2006) Cardiovasc. Res. 70:117.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for SCF/c-kit Ligand Antibody (AB-455-NA)(3)

Publications using AB-455-NA

• Molfetta, R;Lecce, M;Milito, ND;Putro, E;Pietropaolo, G;Marangio, C;Scarno, G;Moretti, M;De Smaele, E;Santini, T;Bernardini, G;Sciumè, G;Santoni, A;Paolini, R; SCF and IL-33 regulate mouse mast cell phenotypic and functional plasticity supporting a pro-inflammatory microenvironment Cell death & disease 2023-09-20 [PMID: 37730723] (Neutralization, Mouse)
• Koung Li Kim, Songyi Seo, Jee Taek Kim, Jaetaek Kim, Won Kim, Yeongju Yeo et al. SCF (Stem Cell Factor) and cKIT Modulate Pathological Ocular Neovascularization Arteriosclerosis, Thrombosis, and Vascular Biology 2019-10-01 [PMID: 31434494]
• Szotek PP, Chang HL, Zhang L, Preffer F, Dombkowski D, Donahoe PK, Teixeira J Adult mouse myometrial label-retaining cells divide in response to gonadotropin stimulation. Stem Cells, 2007-02-08;25(5):1317-25. 2007-02-08 [PMID: 17289934] (IHC-P, Mouse)

Bioinformatics

• Gene Symbol: Kitl
• Uniprot:
  • Q78ED8()