SART1 Antibody - BSA Free

Western Blot: SART1 Antibody [NBP2-14836] - SART1 antibody tested in NIH-3T3 cell lysate.

Immunohistochemistry-Paraffin: SART1 Antibody [NBP2-14836] - SART1 antibody was tested in mouse testes using DAB with hematoxylin counterstain.

• Reactivity: Hu, Mu, Rt
• Applications: WB, IHC, ICC/IF (-)
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.3 mg/ml

SART1 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit SART1 Antibody - BSA Free (NBP2-14836) is a polyclonal antibody validated for use in IHC and WB. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: A synthetic peptide made to a internal portion of the human SART1 protein (between residues 100-200) [UniProt O43290]
• Localization: Nucleus
• Predicted Species: Rat (94%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: SART1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:200-1:500
  • Immunohistochemistry-Paraffin 1:200-1:500
  • Western Blot 1 ug/ml
• Application Notes: This SART1 antibody is useful for IHC-paraffin embedded sections and Western blot. In Western blot a band is detected ~100 kDa in NIH-3T3 cell lysate. In IHC-P, staining was observed in the nuclei of mouse testes. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended.
• Control:

  NIH 3T3 Whole Cell Lysate

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: rat (94%).

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS and 30% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1.3 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for SART1 Antibody - BSA Free

• Allergen Hom s 1
• Ara1
• HAF
• HOMS1
• hSART-1
• hSnu66
• hypoxia-associated factor
• IgE Autoantigen
• MGC2038
• SART1
• SART-1
• SART1(259) protein
• SART1(800) protein
• SART1259
• small nuclear ribonucleoprotein 110kDa (U4/U6.U5)
• SNRNP110
• SNU66 homolog
• Snu66
• squamous cell carcinoma antigen recognised by T cells
• Squamous cell carcinoma antigen recognized by T cells 1
• squamous cell carcinoma antigen recognized by T cells
• U4/U6.U5 tri-snRNP-associated 110 kDa protein
• U4/U6.U5 tri-snRNP-associated protein 1

Background

SART1 (HAF, Ara1, HOMS1, Snu66) is part of the SNU66/SART1 family. This protein is subject to phosphorylation, and sumoylation by SUMO2. It is highly expressed in the nucleus of highly proliferative cells, but is also expressed in the cytosol of epithelial cancers. HAF is a transcription factor that enhances hypoxia inducible factor 1 (HIF-1)-induced activation in VEGF and EPO genes under hypoxic conditions (http://www.aacrmeetingabstracts.org/cgi/content/abstract/2006/1/409-d). SART1/HAF is up-regulated in a multitude of tumors. During long periods of hypoxia, its level increases, but the levels are decreased during acute hypoxia. A recent study showed that SART1/HAF is an E3 ubiquitin ligase that binds and ubiquitinates HIF-1 alpha by an oxygen and pVHL-independent mechanism, which then targets HIF-1 alpha for proteasomal degradation. SART1/HAF also binds HIF-2 alpha at a separate site and increases HIF-2 alpha transactivation without triggering degradation. Therefore, HAF is able to alter the response of cancer cells from HIF-1 alpha-dependent to HIF-2 alpha-dependent transcription and initiates genes involved in invasion such as MMP9, PAI-1, and the stem cell factor OCT-3/4. The shift to HIF-2 alpha dependent gene expression instigated by HAF also promotes an enhanced tumor stem cell population, which results in extremely aggressive tumors in vivo. SART1/HAF presents a method for more aggressive tumor growth under sustained hypoxia (PMID: 21512133; 18838541; 19377289)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: SART1
• Entrez:
  • 9092(Human)
  • 20227(Mouse)
• Uniprot:
  • O43290()
  • Q9Z315(Mouse)