SART1 Antibody

Images

 
Western Blot: SART1 Antibody [NBP2-14836] - SART1 antibody tested in NIH-3T3 cell lysate.
Immunohistochemistry-Paraffin: SART1 Antibody [NBP2-14836] - SART1 antibody was tested in mouse testes using DAB with hematoxylin counterstain.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, IHC, IHC-P, ICC/IF (-)
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.3 mg/ml

Order Details

SART1 Antibody Summary

Immunogen
A synthetic peptide made to a internal portion of the human SART1 protein (between residues 100-200) [UniProt O43290]
Localization
Nucleus
Predicted Species
Rat (94%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
SART1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1 ug/ml
  • Immunohistochemistry 1:200-1:500
  • Immunohistochemistry-Paraffin 1:200-1:500
Application Notes
This SART1 antibody is useful for IHC-paraffin embedded sections and Western blot. In Western blot a band is detected ~100 kDa in NIH-3T3 cell lysate. In IHC-P, staining was observed in the nuclei of mouse testes. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended.
Positive Control
NIH 3T3 Lysate (NB800-PC8)

Reactivity Notes

Human and mouse. Immunogen sequence has 94% homology to rat.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS and 30% Glycerol
Preservative
0.05% Sodium Azide
Concentration
1.3 mg/ml
Purity
Immunogen affinity purified

Alternate Names for SART1 Antibody

  • Allergen Hom s 1
  • Ara1
  • HAF
  • HOMS1
  • hSART-1
  • hSnu66
  • hypoxia-associated factor
  • IgE Autoantigen
  • MGC2038
  • SART1
  • SART-1
  • SART1(259) protein
  • SART1(800) protein
  • SART1259
  • small nuclear ribonucleoprotein 110kDa (U4/U6.U5)
  • SNRNP110
  • SNU66 homolog
  • Snu66
  • squamous cell carcinoma antigen recognised by T cells
  • Squamous cell carcinoma antigen recognized by T cells 1
  • squamous cell carcinoma antigen recognized by T cells
  • U4/U6.U5 tri-snRNP-associated 110 kDa protein
  • U4/U6.U5 tri-snRNP-associated protein 1

Background

SART1 (HAF, Ara1, HOMS1, Snu66) is part of the SNU66/SART1 family. This protein is subject to phosphorylation, and sumoylation by SUMO2. It is highly expressed in the nucleus of highly proliferative cells, but is also expressed in the cytosol of epithelial cancers. HAF is a transcription factor that enhances hypoxia inducible factor 1 (HIF-1)-induced activation in VEGF and EPO genes under hypoxic conditions (http://www.aacrmeetingabstracts.org/cgi/content/abstract/2006/1/409-d). SART1/HAF is up-regulated in a multitude of tumors. During long periods of hypoxia, its level increases, but the levels are decreased during acute hypoxia. A recent study showed that SART1/HAF is an E3 ubiquitin ligase that binds and ubiquitinates HIF-1 alpha by an oxygen and pVHL-independent mechanism, which then targets HIF-1 alpha for proteasomal degradation. SART1/HAF also binds HIF-2 alpha at a separate site and increases HIF-2 alpha transactivation without triggering degradation. Therefore, HAF is able to alter the response of cancer cells from HIF-1 alpha-dependent to HIF-2 alpha-dependent transcription and initiates genes involved in invasion such as MMP9, PAI-1, and the stem cell factor OCT-3/4. The shift to HIF-2 alpha dependent gene expression instigated by HAF also promotes an enhanced tumor stem cell population, which results in extremely aggressive tumors in vivo. SART1/HAF presents a method for more aggressive tumor growth under sustained hypoxia (PMID: 21512133; 18838541; 19377289)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

NB100-64775
Species: Hu, Bb, Bv, Pm
Applications: ELISA, Flow, Func, ICC/IF, IHC, IHC-Fr, IP
NBP1-87962
Species: Hu
Applications: ICC/IF, IHC, IHC-P
NB100-74648
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P, IP
NBP1-81853
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
NBP2-45719
Species: Hu
Applications: WB, IHC
NB600-470
Species: Ye
Applications: WB, ELISA
H00003126-M01
Species: Hu
Applications: WB, ELISA, IHC-P
NBP2-22273
Species: Hu, Mu
Applications: WB, IHC-P, IP
NB100-56618
Species: Hu
Applications: WB, Flow, ICC/IF, IHC, IHC-P
NBP2-24651
Species: Hu, Mu, Eq, Pm
Applications: WB
NBP1-81653
Species: Hu
Applications: ICC/IF, IHC, IHC-P
NBP1-85358
Species: Hu, Mu, Rt
Applications: WB, Simple Western, ICC/IF, IHC, IHC-P

Publications for SART1 Antibody (NBP2-14836) (0)

There are no publications for SART1 Antibody (NBP2-14836).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for SART1 Antibody (NBP2-14836) (0)

There are no reviews for SART1 Antibody (NBP2-14836). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen
  • 1st to review with an image -- $50/€35/£30/$50 CAD/¥300 Yuan/¥5000 Yen

Product General Protocols

View specific protocols for SART1 Antibody (NBP2-14836): Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for SART1 Antibody (NBP2-14836) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Additional SART1 Products

Bioinformatics Tool for SART1 Antibody (NBP2-14836)

Discover related pathways, diseases and genes to SART1 Antibody (NBP2-14836). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for SART1 Antibody (NBP2-14836)

Discover more about diseases related to SART1 Antibody (NBP2-14836).
 

Pathways for SART1 Antibody (NBP2-14836)

View related products by pathway.

PTMs for SART1 Antibody (NBP2-14836)

Learn more about PTMs related to SART1 Antibody (NBP2-14836).
 

Research Areas for SART1 Antibody (NBP2-14836)

Find related products by research area.

Blogs on SART1

There are no specific blogs for SART1, but you can read our latest blog posts.

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our SART1 Antibody and receive a gift card or discount.

Bioinformatics

Gene Symbol SART1
Entrez
Uniprot