Sarcalumenin Antibody (XIIC4)

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Product Details

Summary
Reactivity Mu, Ca, GP, RbSpecies Glossary
Applications WB, IF
Clone
XIIC4
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

Sarcalumenin Antibody (XIIC4) Summary

Immunogen
Purified rabbit sarcoplasmic reticulum membrane vesicles.
Specificity
This specifically detects sarcalumenin and the 53 kDa glycoprotein splice variant.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
SRL
Purity
Protein A purified
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Applications/Dilutions

Dilutions
  • Western Blot 1:500
  • Immunofluorescence 1:500
Application Notes
WB: Detects an approx. 53 kDa and a 160 kDa protein representing the glycoprotein and sarcalumenin respectively, in rabbit skeletal muscle extracts. IF: Staining of sarcalumenin and its splice variant in rabbit skeletal muscle results in staining that co-localizes with calcium ATPase in the longitudinal sarcoplasmic reticulum (SR) and the terminal cisternae.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Ascites with PBS
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Purity
Protein A purified

Alternate Names for Sarcalumenin Antibody (XIIC4)

  • sarcalumenin

Background

Sarcalumenin is a 160 kDa acidic calcium binding glycoprotein found to reside at the inner membrane of the sarcoplasmic reticulum in a calcium dependent manner.The gene encoding sarcalumenin also encodes an alternate splice variant, a 53 kDa glycoprotein which co-localizes with sarcalumenin. These two proteins are found in the longitudinal sarcoplasmic reticulum and the nonjunctional membranes of the terminal cisternae. They have been observed to co-localize with calcium-ATPase suggesting that they are involved in calcium transport and sequestration.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol SRL
Entrez