SAP130 Antibody (JG63-53)

Images

 
Western Blot: SAP130 Antibody (JG63-53) [NBP2-76869] - Western blot analysis of SF3B3 on Jurkat and Hela cells lysates using anti-SF3B3 antibody at 1/1,000 dilution.
Immunocytochemistry/ Immunofluorescence: SAP130 Antibody (JG63-53) [NBP2-76869] - ICC staining SF3B3 (green) in A549 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised ...read more
Immunohistochemistry: SAP130 Antibody (JG63-53) [NBP2-76869] - Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-SF3B3 antibody. Counter stained with hematoxylin.
Flow Cytometry: SAP130 Antibody (JG63-53) [NBP2-76869] - Flow cytometric analysis of LOVO cells with SF3B3 antibody at 1/100 dilution (yellow) compared with an unlabelled control (cells without incubation with primary ...read more
Immunohistochemistry: SAP130 Antibody (JG63-53) [NBP2-76869] - Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-SF3B3 antibody. Counter stained with hematoxylin.
Immunohistochemistry: SAP130 Antibody (JG63-53) [NBP2-76869] - Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-SF3B3 antibody. Counter stained with hematoxylin.
Immunohistochemistry: SAP130 Antibody (JG63-53) [NBP2-76869] - Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-SF3B3 antibody. Counter stained with hematoxylin.
Immunohistochemistry: SAP130 Antibody (JG63-53) [NBP2-76869] - Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-SF3B3 antibody. Counter stained with hematoxylin.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, Flow, ICC/IF, IHC, IHC-P
Clone
JG63-53
Clonality
Monoclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

SAP130 Antibody (JG63-53) Summary

Additional Information
Recombinant Monoclonal Antibody.
Immunogen
Recombinant protein within the C-terminus of human SF3B3.
Localization
Nucleus. Spliceosome.
Isotype
IgG
Clonality
Monoclonal
Host
Rabbit
Gene
SF3B3
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:1000
  • Flow Cytometry 1:50-1:100
  • Immunocytochemistry/Immunofluorescence 1:50-1:100
  • Immunohistochemistry 1:50-1:200
  • Immunohistochemistry-Paraffin 1:50-1:200
Theoretical MW
136 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
TBS (pH7.4), 1%BSA, 40% Glycerol
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Purity
Protein A purified

Alternate Names for SAP130 Antibody (JG63-53)

  • KIAA0017splicing factor 3b, subunit 3, 130kD
  • Pre-mRNA-splicing factor SF3b 130 kDa subunit
  • RSE1
  • SAP130SAP 130
  • SF3B130
  • SF3b130pre-mRNA splicing factor SF3b, 130 kDa subunit
  • Spliceosome-associated protein 130
  • splicing factor 3B subunit 3
  • splicing factor 3b, subunit 3, 130kDa
  • STAF130

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol SF3B3