SAP102 Antibody (2B6) - Azide and BSA Free

Western Blot: SAP102 Antibody (2B6) [H00001741-M03] - Analysis of DLG3 expression in transfected 293T cell line by DLG3 monoclonal antibody (M03), clone 2B6. Lane 1: DLG3 transfected lysatE (90.3 KDa). Lane 2: Non-transfected lysate.

Western Blot: SAP102 Antibody (2B6) [H00001741-M03] - Western blot analysis of DLG3 over-expressed 293 cell line, cotransfected with DLG3 Validated Chimera RNAi or non-transfected control. Blot probed with H00001741-M03. GAPDH (36.1 kDa) used as loading control.

• Reactivity: Hu
• Applications: WB, ELISA, KD
• Clone: 2B6
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

SAP102 Antibody (2B6) - Azide and BSA Free Summary

• Description: Quality control test: Antibody Reactive Against Recombinant Protein.
• Immunogen: DLG3 (NP_066943, 281 a.a. ~ 380 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. VNNTNLQDVRHEEAVASLKNTSDMVYLKVAKPGSLHLNDMYAPPDYASTFTALADNHISHNSSLGYLGAVESKVSYPAPPQVPPTRYSPIPRHMLAEEDF
• Specificity: DLG3 (2B6)
• Isotype: IgG2a Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: DLG3
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Knockdown Validated
  • Western Blot 1:500RNAi Validation
• Application Notes: Antibody reactivity against Recombinant Protein with GST tag on ELISA and WB. GST tag alone is used as a negative control. It has been used for RNAi Validation.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for SAP102 Antibody (2B6) - Azide and BSA Free

• discs, large homolog 3 (Drosophila)
• KIAA1232discs, large homolog 3 (neuroendocrine-dlg, Drosophila)
• MRX90MRX
• NEDLGdisks large homolog 3
• neuroendocrine-DLG
• SAP-102
• SAP102NE-Dlg
• Synapse-associated protein 102
• XLMR

Background

Synapse-Associated Protein 102 (SAP102) is one of a family of plasma membrane-associated proteins found in synaptic junctions. Like other members of the family, SAP102 has three ~90 amino acid repeats called PDZ domains followed by an SH3 domain and a yeast guanylate kinase homology (GuK) domain. It is hypothesized that PDZ-domain interactions play a role in receptor and channel clustering which contributes to neuronal plasticity. SAP102 is believed to participate in the clustering of certain proteins, including NMDA receptors, shaker-type potassium channels at the synaptic membrane in CNS neurons. NMDA receptors and Shaker-type potassium channels both share C-terminal sequence homology consisting of a threonine/serine-X-valine-COOH (T/SXV) motif. Other neuronal proteins that share this motif (beta 1 adrenergic receptor, some serotonin receptors, some sodium channel subunits, and additional potassium channel subunits) may interact with SAP102 by binding to its PDZ domains. Neuronal nitric oxide synthase (nNOS), which lacks the T/SXV motif but which has its own PDZ domain, has been shown to associate with SAP102 in vitro through a pseudo-homotypic PDZ-PDZ interaction.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: DLG3
• Entrez:
  • 1741(Human)
• OMIM:
  • 300189(Human)
• Uniprot:
  • NP_066943(Human)