SAMSN1 Antibody (OTI7F5) - Azide and BSA Free

Western Blot: SAMSN1 Antibody (OTI7F5) - Azide and BSA Free [NBP2-73984] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SAMSN1 .

Immunohistochemistry: SAMSN1 Antibody (OTI7F5) - Azide and BSA Free [NBP2-73984] - Analysis of Human lymphoma tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)(1:150)

Immunohistochemistry: SAMSN1 Antibody (OTI7F5) - Azide and BSA Free [NBP2-73984] - Analysis of Human tonsil tissue.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)(1:150)

• Reactivity: Hu
• Applications: WB, IHC
• Clone: OTI7F5
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: LYOPH

SAMSN1 Antibody (OTI7F5) - Azide and BSA Free Summary

• Immunogen: Human recombinant protein fragment corresponding to amino acids 1-250 of human SAMSN1(NP_071419) produced in E.coli.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: SAMSN1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:150
  • Immunohistochemistry-Paraffin
  • Western Blot 1:2000
• Theoretical MW: 41.5 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Lyophilized from PBS (pH 7.3) with 8% Trehalose
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Immunogen affinity purified
• Reconstitution Instructions: we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Alternate Names for SAMSN1 Antibody (OTI7F5) - Azide and BSA Free

• HACS1gene with homology to KIAA0790 protein10NASH1
• Hematopoietic adaptor containing SH3 and SAM domains 1
• SAM and SH3 domain containing 1
• SAM and SH3 domain containing 2
• SAM domain, SH3 domain and nuclear localisation signals, 1
• SAM domain, SH3 domain and nuclear localization signals 1
• SAM domain, SH3 domain and nuclear localization signals protein 1
• SAM domain-containing protein SAMSN-1
• SH3D6B
• SH3-SAM adaptor protein

Background

SAMSN1 is a member of a novel gene family of putative adaptors and scaffold proteins containing SH3 and SAM (sterile alpha motif) domains. It also contains several predicted consensus nuclear localization signals and a tyrosine kinase phosphorylation motif. Northern blot analysis has revealed expression of a 2.2kb SAMSN1 transcript in human immune tissues and hematopoietic cell types including normal bone marrow, acute myeloid leukemia, and multiple myeloma; low-level expression was seen in other tissues such as heart, brain, placenta, and lung. SAMSN1 has been shown to be up-regulated by B cell activation signals and is a participant in B cell activation and differentiation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: SAMSN1