SAFB Antibody (5A11)

Product Discontinued

SAFB Antibody (5A11) Summary

• Immunogen: SAFB (NP_002958, 111 a.a. - 201 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
• Isotype: IgG2b Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: SAFB
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100 - 1:2000
  • Immunofluorescence 10 ug/ml
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 5 ug/ml
  • Western Blot 1:100-1:2000

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.4)
• Preservative: No Preservative
• Concentration: 0.5 mg/ml
• Purity: Protein A purified

Alternate Names for SAFB Antibody (5A11)

• glutathione S-transferase fusion protein
• HAP
• heat-shock protein (HSP27) estrogen response element and TATA box-bindingprotein
• HETDKFZp779C1727
• Hsp27 ERE-TATA binding protein
• HSP27 ERE-TATA-binding protein
• HSP27 estrogen response element-TATA box-binding protein
• SAF-B
• SAF-B1
• SAFB1SAB-B1
• scaffold attachment factor B
• scaffold attachment factor B1

Background

Activity of the estrogen receptor (ER) is regulated through interaction with coactivators and corepressors. These proteins are present in large complexes, suggesting functional interactions among various cofactors. The scaffold attachment factor SAFB2, a component of high molecular weight nuclear protein complexes which contains a core set of protein interaction partners, functions as an estrogen receptor corepressor. It directly interacts with ER alpha repressing transcription via repression domains. Its over expression results in inhibition of proliferation. SAFB2 is found in the cytoplasm, as well as the nucleus. Consistent with its cytoplasmic localization, there is also an interaction between SAFB2 and vinexin, a protein involved in linking signaling to the cytoskeleton.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: SAFB