S1P5/EDG-8 Antibody (1196A) [Unconjugated]

Western blot shows lysates of HEK293 human embryonic kidney cell line either mock transfected or transfected with mouse S1P5. PVDF membrane was probed with 1 µg/mL of Rabbit Anti-Mouse S1P5/EDG-8 Monoclonal Antibody (Catalog # MAB9084) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for S1P5/EDG-8 at approximately 75 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

HEK293 human embryonic kidney cell line transfected with mouse S1P5/EDG-8 and eGFP were stained with and either (A) Rabbit Anti-Mouse S1P5/EDG-8 Monoclonal Antibody (Catalog # MAB9084) or (B) Normal Rabbit IgG Control (Catalog # AB-105-C) followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B). View our protocol for Staining Membrane-associated Proteins.

• Reactivity: Mu
• Applications: WB, Flow
• Clone: 1196A
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated

S1P5/EDG-8 Antibody (1196A) [Unconjugated] Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Mouse S1P₅/EDG-8 peptide corresponding to the N-terminal extracellular sequence
  Accession # Q91X56
• Specificity: Detects mouse S1P₅/EDG-8 in Western blots.
• Source: N/A
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: S1pr5
• Purity Statement: Protein A or G purified from cell culture supernatant

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 0.25 ug/10^6 cells
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: Sodium Azide
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for S1P5/EDG-8 Antibody (1196A) [Unconjugated]

• EDG8
• Edg-8
• EDG8S1P receptor Edg-8
• Endothelial differentiation G-protein-coupled receptor 8
• endothelial differentiation, sphingolipid G-protein-coupled receptor, 8
• LPB4
• S1P receptor 5
• S1P5
• S1PR5
• sphingosine 1-phosphate receptor 5
• sphingosine 1-phosphate receptor EDG8
• Sphingosine 1-phosphate receptor Edg-8
• sphingosine-1-phosphate receptor 5
• SPPR-1
• SPPR-2

Background

S1P₅ (Sphingosine-1 Phosphate receptor 5), also known as EDG-8 (Endothelial Differentiation Gene 8), is a 43-45 kDa member of the EDG family, S1P-binding subfamily of GPCRs. Along with S1P₁ -S1P₄ (or EDG-1, EDG-5, EDG-3 and EDG-6, respectively), S1P₅ is known to bind S1P, a lipid synthesized by platelets, neutrophils, smooth muscle cells, mast cells and select fibroblasts. Mouse S1P₅ is a 400 amino acid (aa) 7-transmembrane glycoprotein that is expressed on brain endothelium, renal mesangial cells, Ly6C⁺ (in human CD14⁺CD16^-) monocytes, pre- and mature oligodendrocytes, and CD27^-CD116⁺ (mature) NK cells. S1P₅ appears to play a role in cell trafficking. On monocytes and NK cells, S1P₅ promotes cell migration out of the bone marrow, a phenomenon that may not be related to an S1P concentration gradient. S1P₅ also helps maintain the integrity of the blood-brain-barrier. On oligodendrocytes and precursors, S1P₅ appears to regulate cell survival and pseudopod extension. The extracellular segment of mouse S1P₅ shares 96% and 94% aa sequence identity with rat and human S1P₅ extracellular domains, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: S1pr5
• Uniprot:
  • Q91X56()