S1P3/EDG-3 Overexpression Lysate (Native)


Western Blot: EDG3 Overexpression Lysate (Adult Normal) [NBL1-15667] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for EDG3.

Product Details

Applications WB
The exact concentration of the protein of interest cannot be determined for overexpression lysates. Please contact technical support for more information.

Order Details

S1P3/EDG-3 Overexpression Lysate (Native) Summary

The lysate was created in HEK293T cells, using plasmid ID RC209253 and based on accession number NM_005226. The protein contains a C-terminal DDK tag.
Homo sapiens sphingosine-1-phosphate receptor 3 (S1PR3), mRNA.

Packaging, Storage & Formulations

Store at -80C. Avoid freeze-thaw cycles.
RIPA buffer
The exact concentration of the protein of interest cannot be determined for overexpression lysates. Please contact technical support for more information.


Application Notes
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
Theoretical MW
42.1 kDa

Lysate Details for S1P3/EDG-3

Protein State


HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for S1P3/EDG-3 Overexpression Lysate (Native)

  • EDG3
  • EDG-3
  • EDG3FLJ37523
  • Endothelial differentiation G-protein coupled receptor 3
  • endothelial differentiation, sphingolipid G-protein-coupled receptor, 3
  • FLJ93220
  • G protein-coupled receptor, endothelial differentiation gene-3
  • LPB3
  • MGC71696
  • S1P receptor 3
  • S1P receptor EDG3
  • S1P receptor Edg-3
  • S1P3
  • S1PR3
  • sphingosine 1-phosphate receptor 3
  • Sphingosine 1-phosphate receptor Edg-3
  • sphingosine-1-phosphate receptor 3


This gene encodes a member of the EDG family of receptors, which are G protein-coupled receptors. This protein has been identified as a functional receptor for sphingosine 1-phosphate and likely contributes to the regulation of angiogenesis and vascular endothelial cell function. [provided by RefSeq]. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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Publications for S1P3/EDG-3 Lysate (NBL1-15667) (0)

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Gene Symbol S1PR3

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