| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-24762SS | |||
MAB003) or (B) Mouse Anti-Human S1P3/EDG-3 Monoclonal Antibody (Catalog # MAB7524) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B)." alt="Human S1P3/EDG-3 and eGFP co-transfectants were stained with either (A) Mouse IgG2A Isotype Control (Catalog # MAB003) or (B) Mouse Anti-Human S1P3/EDG-3 Monoclonal Antibody (Catalog # MAB7524) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B)." class="big_thumb" />
CTS013). Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to the marginal zone of germinal centers. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." alt="S1P3/EDG-3 was detected in immersion fixed paraffin-embedded sections of human spleen using Mouse Anti-Human S1P3/EDG-3 Monoclonal Antibody (Catalog # MAB75241) at 15 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to the marginal zone of germinal centers. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="big_thumb" />
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB75241-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-22313-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-22220-50ul | |||
![Western Blot: S1P3/EDG-3 Antibody [NBP2-24762] - Human neuroblastoma with various drugs and control. Image from verified customer review.](http://images.novusbio.com/fullsize/S1P3-EDG-3-Antibody-Western-Blot-NBP2-24762-img0007.jpg "Western Blot: S1P3/EDG-3 Antibody [NBP2-24762] - Human neuroblastoma with various drugs and control. Image from verified customer review.")
![Immunohistochemistry-Paraffin: S1P3/EDG-3 Antibody [NBP2-24762] - Human liver tissue using an isotype control (top left) and NBP2-24762 (bottom left, right) at 5 ug/ml.](http://images.novusbio.com/fullsize/S1P3-EDG-3-Antibody-Immunohistochemistry-Paraffin-NBP2-24762-img0006.jpg)
![Western Blot: S1P3/EDG-3 Antibody [NBP2-24762] - Sphingolipid Receptor Edg3/S1P3 in kidney lysate. Lane 1 shows pre-immune sera. Lanes 2 and 3 show NBP2-24762 tested on human kidney (2 ug/ml) and mouse kidney (1 ug/ml) lysate.](http://images.novusbio.com/fullsize/S1P3-EDG-3-Antibody-Western-Blot-NBP2-24762-img0005.jpg)
![Western Blot: S1P3/EDG-3 Antibody (5E3G9) [NBP3-15479] - Western blot analysis of extracts of various cell lines, using S1P3/EDG-3 Rabbit mAb (NBP3-15479) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.](http://images.novusbio.com/fullsize/S1P3-EDG-3-Antibody-5E3G9-Western-Blot-NBP3-15479-img0001.jpg "Western Blot: S1P3/EDG-3 Antibody (5E3G9) [NBP3-15479] - Western blot analysis of extracts of various cell lines, using S1P3/EDG-3 Rabbit mAb (NBP3-15479) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.")
![Western Blot: S1P3/EDG-3 Antibody (SR1706) [NBP3-22313] - Western blot analysis using NBP3-22313 in HepG2 cell lysate.](http://images.novusbio.com/fullsize/nbp3-22313_rabbit-s1p3-edg-3-mab-sr1706-672023106243.jpg "Western Blot: S1P3/EDG-3 Antibody (SR1706) [NBP3-22313] - Western blot analysis using NBP3-22313 in HepG2 cell lysate.")
![Immunohistochemistry-Paraffin: S1P3/EDG-3 Antibody [NBP3-14467] - Immunohistochemistry of formalin-fixed, paraffin-embedded human ovary carcinoma after heat-induced antigen retrieval.](http://images.novusbio.com/fullsize/S1P3-EDG-3-Antibody-Immunohistochemistry-Paraffin-NBP3-14467-img0002.jpg "Immunohistochemistry-Paraffin: S1P3/EDG-3 Antibody [NBP3-14467] - Immunohistochemistry of formalin-fixed, paraffin-embedded human ovary carcinoma after heat-induced antigen retrieval.")
![Immunohistochemistry-Paraffin: S1P3/EDG-3 Antibody [NBP3-14467] - Immunohistochemistry of formalin-fixed, paraffin-embedded human prostate carcinoma after heat-induced antigen retrieval.](http://images.novusbio.com/fullsize/S1P3-EDG-3-Antibody-Immunohistochemistry-Paraffin-NBP3-14467-img0001.jpg)
![Immunohistochemistry-Paraffin: S1P3/EDG-3 Antibody [NBP3-14467] - Immunohistochemistry of formalin-fixed, paraffin-embedded human kidney after heat-induced antigen retrieval.](http://images.novusbio.com/fullsize/S1P3-EDG-3 Antibody-Immunohistochemistry-Paraffin-NBP3-14467-img0003.jpg)
![ELISA: Mouse S1P3/EDG-3 ELISA Kit (Colorimetric) [NBP2-82428] - Samples were spiked with high concentrations of Mouse S1P3/EDG-3 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.](http://images.novusbio.com/fullsize/nbp2-82428_mouse-s1p3-edg-3-elisa-kit-colorimetric-132202416121359.jpg "ELISA: Mouse S1P3/EDG-3 ELISA Kit (Colorimetric) [NBP2-82428] - Samples were spiked with high concentrations of Mouse S1P3/EDG-3 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.")
![ELISA: Mouse S1P3/EDG-3 ELISA Kit (Colorimetric) [NBP2-82428] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp2-82428_mouse-s1p3-edg-3-elisa-kit-colorimetric-43202498356.jpg)
![Western Blot: S1P3/EDG-3 Antibody (SR1721) [NBP3-22220] - Western blot analysis using NBP3-22220 in HepG2 cell lysate.](http://images.novusbio.com/fullsize/nbp3-22220_rabbit-s1p3-edg-3-mab-sr1721-57202315584833.jpg "Western Blot: S1P3/EDG-3 Antibody (SR1721) [NBP3-22220] - Western blot analysis using NBP3-22220 in HepG2 cell lysate.")
![SDS-Page: Recombinant Human S1P3/EDG-3 Protein [H00001903-Q01] - 12.5% SDS-PAGE Stained with Coomassie Blue.](http://images.novusbio.com/fullsize/qc_test-H00001903-Q01-1.jpg "SDS-Page: Recombinant Human S1P3/EDG-3 Protein [H00001903-Q01] - 12.5% SDS-PAGE Stained with Coomassie Blue.")
![Western Blot: EDG3 Overexpression Lysate (Adult Normal) [NBL1-15667] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for EDG3.](http://images.novusbio.com/fullsize/EDG3-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-15667-img0002.jpg "Western Blot: EDG3 Overexpression Lysate (Adult Normal) [NBL1-15667] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for EDG3.")
![Western Blot: S1P3/EDG-3 Antibody [NBP3-09489] - Western blot analysis of S1P3/EDG-3 in Hela Whole Cell as a positive control. Antibody dilution at 1.0 ug/ml](http://images.novusbio.com/fullsize/S1P3-EDG-3-Antibody-Western-Blot-NBP3-09489-img0001.jpg "Western Blot: S1P3/EDG-3 Antibody [NBP3-09489] - Western blot analysis of S1P3/EDG-3 in Hela Whole Cell as a positive control. Antibody dilution at 1.0 ug/ml")
