Mouse S1P3/EDG-3 ELISA Kit (Colorimetric)

ELISA: Mouse S1P3/EDG-3 ELISA Kit (Colorimetric) [NBP2-82428] - Samples were spiked with high concentrations of Mouse S1P3/EDG-3 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

ELISA: Mouse S1P3/EDG-3 ELISA Kit (Colorimetric) [NBP2-82428] - Standard Curve Reference

• Reactivity: Mu
• Applications: ELISA
• Suitable Sample Type: Serum, plasma, cell supernatant, and other biological fluids
• Standard Curve Range: 62.5 - 4000 pg/mL
• Sensitivity: 37.5 pg/mL

Mouse S1P3/EDG-3 ELISA Kit (Colorimetric) Summary

• Specificity: This kit recognizes Mouse S1P3/EDG-3 in samples. No Significant cross-reactivity or interference between Mouse S1P3/EDG-3 and analogues was observed.
• Standard Curve Range: 62.5 - 4000 pg/mL
• Sensitivity: 37.5 pg/mL
• Assay Type: Sandwich-ELISA
• Inter-Assay: CV% < 5.6
• Intra-Assay: CV% < 5.12
• Spike Recovery: 84-103%
• Sample Volume: 100 ul
• Kit Type: ELISA Kit (Colorimetric)
• Gene: S1PR3

Applications/Dilutions

• Dilutions:
  • ELISA

Packaging, Storage & Formulations

• Storage: Storage of components varies. See protocol for specific instructions.

Alternate Names for Mouse S1P3/EDG-3 ELISA Kit (Colorimetric)

• EDG3
• EDG-3
• EDG3FLJ37523
• Endothelial differentiation G-protein coupled receptor 3
• endothelial differentiation, sphingolipid G-protein-coupled receptor, 3
• FLJ93220
• G protein-coupled receptor, endothelial differentiation gene-3
• LPB3
• MGC71696
• S1P receptor 3
• S1P receptor EDG3
• S1P receptor Edg-3
• S1P3
• S1PR3
• sphingosine 1-phosphate receptor 3
• Sphingosine 1-phosphate receptor Edg-3
• sphingosine-1-phosphate receptor 3

Background

EDG3, a Lysophospholipid/Lysosphingolipid Receptor, binds the vasoconstrictor sphingosine-1-phosphate and activates transcription of serum response element-driven reporter genes. EDG3 may regulate angiogenesis and vascular endothelial cell functions. EDG3 has been reported in all tissues, with highest levels in heart, placenta, kidney, and liver. ESTs have been isolated from placenta and germ cell tumor libraries.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

Publications for S1P3/EDG-3 ELISA Kit (NBP2-82428)(1)

Publication using NBP2-82428

• Lin R, Wang J, Wu Y et al. Resolving neutrophils due to TRAM deletion renders protection against experimental sepsis Inflammation research : official journal of the European Histamine Research Society ... [et al.] 2023-08-10 [PMID: 37563334] (ELISA)

FAQs for S1P3/EDG-3 ELISA Kit (NBP2-82428). (Showing 1 - 1 of 1 FAQ).

1. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.

Bioinformatics

• Gene Symbol: S1PR3