S1P2/EDG-5/S1PR2 Antibody (HL3765)

Unboiled various tissue extracts (50 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with S1P2/EDG-5/S1PR2 antibody [HL3765] (NBP3-48844) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data are based on NCBI database.

Unboiled various tissue extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with S1P2/EDG-5/S1PR2 antibody [HL3765] (NBP3-48844) diluted at 1:2400. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data are based on NCBI database.

Unboiled Raji whole cell and membrane extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with S1P2/EDG-5/S1PR2 antibody [HL3765] (NBP3-48844) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody

Boiled and unboiled HCT116 whole cell and membrane extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with S1P2/EDG-5/S1PR2 antibody [HL3765] (NBP3-48844) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody

Unboiled various tissue extracts (50 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with S1P2/EDG-5/S1PR2 antibody [HL3765] (NBP3-48844) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

• Reactivity: Hu, Mu, Rt
• Applications: WB
• Clone: HL3765
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated

S1P2/EDG-5/S1PR2 Antibody (HL3765) Summary

• Description: Novus Biologicals Rabbit S1P2/EDG-5/S1PR2 Antibody (HL3765) (NBP3-48844) is a recombinant monoclonal antibody validated for use in WB. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: Synthetic peptide encompassing a sequence within the Intracellular domain of human S1P2/EDG-5/S1PR2. The exact sequence is proprietary.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: S1PR2
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Western Blot

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: No Preservative
• Purity: Protein A purified

Alternate Names for S1P2/EDG-5/S1PR2 Antibody (HL3765)

• AGR16
• EDG5
• EDG-5
• endothelial differentiation G-protein coupled receptor 5
• endothelial differentiation, sphingolipid G-protein-coupled receptor, 5
• GPCR13
• GPCR18
• H218
• LPB2
• S1P receptor 2
• S1P receptor EDG5
• S1P receptor Edg-5
• S1P2
• S1PR2
• sphingosine 1-phosphate receptor 2
• sphingosine 1-phosphate receptor Edg-5
• sphingosine-1-phosphate receptor 2

Background

This EDG5 protein is a member of the G protein-coupled receptors, as well as the EDG family of proteins. This protein participates in sphingosine 1-phosphate-induced cell proliferation, survival, and transcriptional activation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: S1PR2