S1P1/EDG-1 Antibody (218713) [Unconjugated]

CHO Chinese hamster ovary cell line transfected with either (A) human S1P1/EDG-1 or (B) irrelevant transfectants and eGFP were stained with Mouse Anti-Human S1P1/EDG-1 Monoclonal Antibody (Catalog # MAB2016) followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B). Quadrant markers were set based on control antibody staining (Catalog # MAB0041). View our protocol for Staining Membrane-associated Proteins.

S1PR1 expression by OECs is dynamically altered by microenvironment.OEC expression of S1PR1 under normoxia and hypoxia was qualitatively confirmed by immunocytochemistry (A). The percentage of cells expressing S1PR1 on the cell surface, as confirmed with flow cytometry, was enhanced under hypoxia as compared to the normoxic control (B). Combined stimuli of S1P and VEGF resulted in greater S1PR1 mRNA production after 24h in normoxia (C). Combined stimuli also resulted in a greater proportion of S1PR1+ OECs under both normoxia and hypoxia (D and E). Scale bars represent 40 μm. Data are mean ± SD (n = 4) and normalized to the average untreated control values for either normoxia or hypoxia (C, E; indicated by dashed line) accordingly. An asterisk indicates statistically significant differences (P<0.05) between conditions in normoxia or hypoxia respectively and N.S. displays no statistically significant difference between conditions. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0123437), licensed under a CC-BY license. Not internally tested by R&D Systems.

• Reactivity: Hu
• Applications: Flow, CyTOF-ready
• Clone: 218713
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

S1P1/EDG-1 Antibody (218713) [Unconjugated] Summary

• Additional Information: Clone 218713 was used by HLDA to establish CD designation
• Immunogen: NS0 mouse myeloma cell line transfected with human S1P₁/EDG‑1
  Met1-Ser382
  Accession # P21453
• Specificity: Detects human S1P₁/EDG‑1 by flow cytometry.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: S1PR1
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry 0.25 ug/10^6 cells

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for S1P1/EDG-1 Antibody (218713) [Unconjugated]

• CD363 antigen
• CD363
• CHEDG1
• D1S3362
• EDG1
• EDG-1
• EDG1edg-1
• Endothelial differentiation G-protein coupled receptor 1
• endothelial differentiation, sphingolipid G-protein-coupled receptor, 1
• FLJ58121
• S1P receptor 1
• S1P receptor Edg-1
• S1P1
• S1P1ECGF1
• S1PR1
• sphingosine 1-phosphate receptor 1
• sphingosine 1-phosphate receptor EDG1
• Sphingosine 1-phosphate receptor Edg-1
• sphingosine-1-phosphate receptor 1

Background

S1P₁, also known as EDG-1, is a member of the endothelial differentiation gene family. It is a high affinity G protein-coupled receptor for the bioactive lipid, Sphingosine-1-phosphate. S1P₁ signaling regulates endothelial cell survival, cytoskeletal remodeling, chemotaxis, and angiogenesis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for S1P1/EDG-1 Antibody (MAB2016)(11)

Publications using MAB2016

• Nakamura, S;Yamamoto, R;Matsuda, T;Yasuda, H;Nishinaka, A;Takahashi, K;Inoue, Y;Kuromitsu, S;Shimazawa, M;Goto, M;Narumiya, S;Hara, H; Sphingosine-1-phosphate receptor 1/5 selective agonist alleviates ocular vascular pathologies Scientific reports 2024-04-27 [PMID: 38678148] (Flow Cytometry, Human)
• Johansen, VBI;Hampson, E;Tsonou, E;Pantarelli, C;Chu, JY;Crossland, L;Okkenhaug, H;Massey, AJ;Hornigold, DC;Welch, HCE;Chetwynd, SA; The GPCR adaptor protein Norbin regulates S1PR1 trafficking and the morphology, cell cycle and survival of PC12 cells Scientific reports 2023-10-25 [PMID: 37880240] (Flow Cytometry, Rat)
• Guan-Da Syu, Shih-Chin Wang, Guangzhong Ma, Shuang Liu, Donna Pearce, Atish Prakash et al. Development and application of a high-content virion display human GPCR array Nature Communications 4/30/2019 [PMID: 31040288]
• Linder B, Wehle A, Hehlgans S et al. Arsenic Trioxide and (-)-Gossypol Synergistically Target Glioma Stem-Like Cells via Inhibition of Hedgehog and Notch Signaling Cancers (Basel) 3/12/2019 [PMID: 30871073]
• R Yamamoto, T Aoki, H Koseki, M Fukuda, J Hirose, K Tsuji, K Takizawa, S Nakamura, H Miyata, N Hamakawa, H Kasuya, K Nozaki, Y Hirayama, I Aramori, S Narumiya An S1P1 agonist, ASP4058, suppresses intracranial aneurysm through promoting endothelial integrity and blocking macrophage transmigration Br. J. Pharmacol., 2017-05-27;0(0):. 2017-05-27 [PMID: 28409823] (Flow Cytometry, Human)
• Williams P, Stilhano R, To V, Tran L, Wong K, Silva E Hypoxia augments outgrowth endothelial cell (OEC) sprouting and directed migration in response to sphingosine-1-phosphate (S1P). PLoS ONE, 2015-04-15;10(4):e0123437. 2015-04-15 [PMID: 25875493] (Flow Cytometry, Human)
• Till K, Pettitt A, Slupsky J Expression of functional sphingosine-1 phosphate receptor-1 is reduced by B cell receptor signaling and increased by inhibition of PI3 kinase delta but not SYK or BTK in chronic lymphocytic leukemia cells. J Immunol, 2015-01-28;194(5):2439-46. 2015-01-28 [PMID: 25632006] (Flow Cytometry, Human)
• Gatfield J, Monnier L, Studer R, Bolli M, Steiner B, Nayler O Sphingosine-1-phosphate (S1P) displays sustained S1P1 receptor agonism and signaling through S1P lyase-dependent receptor recycling. Cell Signal, 2014-04-02;26(7):1576-88. 2014-04-02 [PMID: 24704119] (Flow Cytometry, Human)
• Cabezon R, Sintes J, Llinas L, Benitez-Ribas D Analysis of HLDA9 mAbs on plasmacytoid dendritic cells. Immunol. Lett., 2010-10-07;134(2):167-73. 2010-10-07 [PMID: 20933012] (Flow Cytometry, Human)
• Matesanz-Isabel J, Sintes J, Llinas L, de Salort J, Lazaro A, Engel P New B-cell CD molecules. Immunol. Lett., 2010-10-07;134(2):104-12. 2010-10-07 [PMID: 20933010] (Flow Cytometry, Human)
• Lorquet S, Berndt S, Blacher S, Gengoux E, Peulen O, Maquoi E, Noel A, Foidart JM, Munaut C, Pequeux C Soluble forms of VEGF receptor-1 and -2 promote vascular maturation via mural cell recruitment. FASEB J., 2010-05-19;24(10):3782-95. 2010-05-19 [PMID: 20484670] (Western Blot, Human)

Review for S1P1/EDG-1 Antibody (MAB2016) (1)

Average Rating: 3

(Based on 1 review)

We have 1 review tested in 1 species: Canine.

reviewed by: Verified Customer Flow Canine 10/12/2018

Summary

• Application: Flow Cytometry
• Sample Tested: Whole blood cells
• Species: Canine
• Lot: KDK021809A

Bioinformatics

• Gene Symbol: S1PR1
• Uniprot:
  • P21453()