S1P1/EDG-1 Antibody (218713) [Phycoerythrin]

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CHO Chinese hamster ovary cell line transfected with either (A) human S1P₁/EDG-1 or (B) irrelevant transfectants and eGFP were stained with Mouse Anti-Human S1P₁/EDG-1 PE-conjugated Monoclonal Antibody (Catalog # FAB2016P). Quadrant markers were set based on control antibody staining (Catalog # <a class=

• Reactivity: Hu
• Applications: Flow
• Clone: 218713
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Phycoerythrin

S1P1/EDG-1 Antibody (218713) [Phycoerythrin] Summary

• Additional Information: Clone 218713 was used by HLDA to establish CD designation
• Immunogen: NS0 mouse myeloma cell line transfected with human S1P₁/EDG‑1
  Met1-Ser382
  Accession # P21453
• Specificity: Detects human S1P₁/EDG‑1 by flow cytometry.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: S1PR1
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 10 uL/10^6 cells

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.
• Buffer: Supplied in a saline solution containing BSA and Sodium Azide.
• Preservative: Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for S1P1/EDG-1 Antibody (218713) [Phycoerythrin]

• CD363 antigen
• CD363
• CHEDG1
• D1S3362
• EDG1
• EDG-1
• EDG1edg-1
• Endothelial differentiation G-protein coupled receptor 1
• endothelial differentiation, sphingolipid G-protein-coupled receptor, 1
• FLJ58121
• S1P receptor 1
• S1P receptor Edg-1
• S1P1
• S1P1ECGF1
• S1PR1
• sphingosine 1-phosphate receptor 1
• sphingosine 1-phosphate receptor EDG1
• Sphingosine 1-phosphate receptor Edg-1
• sphingosine-1-phosphate receptor 1

Background

S1P₁, also known as EDG-1, is a member of the endothelial differentiation gene family. It is a high affinity G protein-coupled receptor for the bioactive lipid, Sphingosine-1-phosphate. S1P₁ signaling regulates endothelial cell survival, cytoskeletal remodeling, chemotaxis, and angiogenesis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for S1P1/EDG-1 Antibody (FAB2016P)(5)

Publications using FAB2016P

• J Jin, N Xue, Y Liu, R Fu, M Wang, M Ji, F Lai, J Hu, X Wang, Q Xiao, X Zhang, D Yin, L Bai, X Chen, S Rao A novel S1P1 modulator IMMH002 ameliorates psoriasis in multiple animal models Acta Pharm Sin B, 2019-11-14;10(2):276-288. 2019-11-14 [PMID: 32082973] (Flow Cytometry, Hamster)
• King Faisal Yambire, Lorena Fernandez-Mosquera, Robert Steinfeld, Christiane Mühle, Elina Ikonen, Ira Milosevic et al. Mitochondrial biogenesis is transcriptionally repressed in lysosomal lipid storage diseases eLife 2/18/2019 [PMID: 30775969]
• P Annunziata, C Cioni, G Masi, M Tassi, G Marotta, S Severi Fingolimod reduces circulating tight-junction protein levels and in vitro peripheral blood mononuclear cells migration in multiple sclerosis patients Sci Rep, 2018-10-18;8(1):15371. 2018-10-18 [PMID: 30337577] (Flow Cytometry, Human)
• Borge M, Remes Lenicov F, Nannini P, de los Rios Alicandu M, Podaza E, Ceballos A, Fernandez Grecco H, Cabrejo M, Bezares R, Morande P, Oppezzo P, Giordano M, Gamberale R The expression of sphingosine-1 phosphate receptor-1 in chronic lymphocytic leukemia cells is impaired by tumor microenvironmental signals and enhanced by piceatannol and R406. J Immunol, 2014-08-15;193(6):3165-74. 2014-08-15 [PMID: 25127862] (Flow Cytometry, Human)
• Llinas L, Lazaro A, de Salort J, Matesanz-Isabel J, Sintes J, Engel P Expression profiles of novel cell surface molecules on B-cell subsets and plasma cells as analyzed by flow cytometry. Immunol. Lett., 2010-10-15;134(2):113-21. 2010-10-15 [PMID: 20951740] (Flow Cytometry, Human)

Bioinformatics

• Gene Symbol: S1PR1
• Uniprot:
  • P21453()