S100A9 Antibody (372510) [Unconjugated]

Western blot shows lysates of mouse leukocytes. PVDF membrane was probed with 2 µg/mL of Rat Anti-Mouse S100A9 Monoclonal Antibody (Catalog # MAB2065) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (HAF005). A specific band was detected for S100A9 at approximately 14 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Simple Western lane view shows lysates of mouse spleen tissue, loaded at 0.2 mg/mL. A specific band was detected for S100A9 at approximately 19 kDa (as indicated) using 10 µg/mL of Rat Anti-Mouse S100A9 Monoclonal Antibody (Catalog # MAB2065) followed by 1:50 dilution of HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

• Reactivity: Mu
• Applications: WB, Simple Western
• Clone: 372510
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Unconjugated
• Concentration: LYOPH

S100A9 Antibody (372510) [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant mouse S100A9
  Met1-Lys113
  Accession # P31725
• Specificity: Detects mouse S100A9 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant mouse (rm) S100A4, rmS100A8, rmS100A10, rhS100B, or rhS100P is observed.
• Source: N/A
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Rat
• Gene: S100A9
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Simple Western 10 ug/mL
  • Western Blot 2 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for S100A9 Antibody (372510) [Unconjugated]

• CAGBMigration inhibitory factor-related protein 14
• Calgranulin B
• calgranulin-B
• Calprotectin L1H subunit
• CFAGMRP-14,60B8AG
• CGLB
• L1AG
• LIAG
• MAC387
• MIF
• MRP-14
• MRP14Leukocyte L1 complex heavy chain
• NIF
• P14
• protein S100-A9
• S100 calcium binding protein A9 (calgranulin B)
• S100 calcium binding protein A9
• S100 calcium-binding protein A9 (calgranulin B)
• S100 calcium-binding protein A9
• S100A9

Background

S100A9, also known as calgranulin B or migration inhibitory factor-related protein 14 (MRP14), belongs to the S100 family of calcium binding proteins of the EF-hand type. It is highly expressed in myeloid cells and other cell types including keratinocytes, epithelial cells and endothelial cells. S100A9 is a cytosolic protein that can also be released into the extracellular milieu. It is associated with various inflammatory diseases.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for S100A9 Antibody (MAB2065)(11)

Publications using MAB2065

• Y Zhou, J Hann, V Schenten, S Plançon, JL Bueb, F Tolle, S Bréchard Role of S100A8/A9 for Cytokine Secretion, Revealed in Neutrophils Derived from ER-Hoxb8 Progenitors International Journal of Molecular Sciences, 2021-08-17;22(16):. 2021-08-17 [PMID: 34445548] (Western Blot, Mouse)
• N Makthal, K Nguyen, H Do, M Gavagan, P Chandrangs, JD Helmann, RJ Olsen, M Kumaraswam A Critical Role of Zinc Importer AdcABC in Group A Streptococcus-Host Interactions During Infection and Its Implications for Vaccine Development EBioMedicine, 2017-06-02;0(0):. 2017-06-02 [PMID: 28596134] (Western Blot, Mouse)
• Interleukin 6 regulates psoriasiform inflammation-associated thrombosis JCI Insight, 2016-12-08;1(20):e89384. 2016-12-08 [PMID: 27942589] (Western Blot, Mouse)
• Chen X, Zhang L, Zhang I, Liang J, Wang H, Ouyang M, Wu S, da Fonseca A, Weng L, Yamamoto Y, Yamamoto H, Natarajan R, Badie B RAGE expression in tumor-associated macrophages promotes angiogenesis in glioma. Cancer Res, 2014-10-17;74(24):7285-97. 2014-10-17 [PMID: 25326491] (Western Blot, Mouse)
• Gumireddy K, Li A, Kossenkov A, Cai K, Liu Q, Yan J, Xu H, Showe L, Zhang L, Huang Q ID1 promotes breast cancer metastasis by S100A9 regulation. Mol Cancer Res, 2014-06-19;12(9):1334-43. 2014-06-19 [PMID: 24948111] (Western Blot, Human)
• Dessing , Mark C, Tammaro , Alessand, Pulskens , Wilco P, Teske , Gwendoli, Butter , Loes M, Claessen , Nike, van Eijk , Marco, van der Poll , Tom, Vogl , Thomas, Roth , Johannes, Florquin , Sandrine, Leemans , Jaklien The calcium-binding protein complex S100A8/A9 has a crucial role in controlling macrophage-mediated renal repair following ischemia/reperfusion. Kidney Int, 2014-06-18;87(1):85-94. 2014-06-18 [PMID: 24940802] (IHC, Mouse)
• Cabrera S, Fernandez AF, Marino G et al. ATG4B/autophagin-1 regulates intestinal homeostasis and protects mice from experimental colitis. Autophagy. 8/1/2013 [PMID: 23782979]
• Yano , Junko, Kolls , Jay K, Happel , Kyle I, Wormley , Floyd, Wozniak , Karen L, Fidel , Paul L J The Acute Neutrophil Response Mediated by S100 Alarmins during Vaginal Candida Infections Is Independent of the Th17-Pathway. PLoS ONE, 2012-09-25;7(9):e46311. 2012-09-25 [PMID: 23050010] (ELISA Development, IHC-Fr, Mouse)
• Proteomic analysis of the role of S-nitrosoglutathione reductase in lipopolysaccharide-challenged mice. Proteomics, 2012-06-01;12(12):2024-35. 2012-06-01 [PMID: 22623366] (Western Blot, Mouse)
• Dessing MC, Butter LM, Teske GJ S100A8/A9 is not involved in host defense against murine urinary tract infection. PLoS ONE, 2010-10-14;5(10):e13394. 2010-10-14 [PMID: 20976233] (IHC-P, Mouse)
• Guttman-Yassky E, Lowes MA, Fuentes-Duculan J, Zaba LC, Cardinale I, Nograles KE, Khatcherian A, Novitskaya I, Carucci JA, Bergman R, Krueger JG Low expression of the IL-23/Th17 pathway in atopic dermatitis compared to psoriasis. J. Immunol., 2008-11-15;181(10):7420-7. 2008-11-15 [PMID: 18981165] (IHC-Fr, Human)

FAQs for S100A9 Antibody (MAB2065). (Showing 1 - 2 of 2 FAQs).

1. Have any of your S100A9 antibodies been tested for use in neutralization, or blocking assays, on human samples?
   • Unfortunately at this time we have not tested, or received customer feedback on our S100A9 antibodies for use in blocking or neutralizing assays. If you were planning on testing any of our S100A9 antibodies for neutralizing or blocking capabilities, we would recommend our Innovator’s Reward Program. Under the terms of this program we would be happy to provide a credit for a free vial in exchange for new data on this previously untested or reported application. Please submit this data in the form of an online review. Additional <a href="http://www.novusbio.com/support/innovators-reward.html" target="_self">Innovators Reward Program</a> information can be found using this link.
2. We would like to stain paraffin embedded human intestinal tissue for Calprotectin. One question will be which cells express the majority of Calprotectin - invading neutrophils vs epithelium for example. The antibody list you provide is quite extensive. Which antibody would you recommend for our purpose? Which tissue would you recommend as positive control.
   • Nine of our antibodies to Calprotectin have been validated for IHC-P with human tissue, and you can see all of these products. A number of our IHC images were generated using human spleen samples, and as such this may be a good choice of a positive control tissue. We do sell a human spleen slide product, which is suitable for IHC-P. Unfortunately I do not have an in-depth knowledge of Calprotectin, however the image shown for our antibody with catalogue number NBP1-02826 demonstrates clear staining of neutrophils. The following paper also suggests that the protein is abundant in neutrophils: PMID 11435495.

Bioinformatics

• Gene Symbol: S100A9
• Uniprot:
  • P31725()