S100A4 Antibody (922813) [Unconjugated]

Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line and NIH-3T3 mouse embryonic fibroblast cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human S100A4 Monoclonal Antibody (Catalog # MAB4137) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for S100A4 at approximately 11 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

S100A4 was detected in immersion fixed paraffin-embedded sections of human cervical cancer tissue using Mouse Anti-Human S100A4 Monoclonal Antibody (Catalog # MAB4137) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in cancer cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

S100A4 was detected in immersion fixed A549 human lung carcinoma cells unstimulated (lower panel) and stimulated with the EMT Inducing Media Supplement (upper panel; Catalog # CCM017) using Mouse Anti-Human S100A4 Monoclonal Antibody (Catalog # MAB4137) at 1 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and S100A4 knockout HeLa cell line (KO). PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human S100A4 Monoclonal Antibody (Catalog # MAB4137) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for S100A4 at approximately 12 kDa (as indicated) in the parental HeLa cell line, but is not detectable in knockout HeLa cell line. GAPDH (Catalog # MAB5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

• Reactivity: Hu
• Applications: WB, IHC, ICC/IF, KO
• Clone: 922813
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

S100A4 Antibody (922813) [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant human S100A4
  Met1-Ser101
  Accession # P26447
• Specificity: Detects human S100A4 in direct ELISAs.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: S100A4
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry 8-25 ug/mL
  • Immunohistochemistry 8-25 ug/mL
  • Knockout Validated
  • Western Blot 2 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for S100A4 Antibody (922813) [Unconjugated]

• 18A2
• 42A
• Calvasculin
• CAPL
• CAPLS100 calcium binding protein A4 (calcium protein, calvasculin, metastasin
• fibroblast-specific protein-1,42A
• FSP1
• leukemia multidrug resistance associated protein
• malignant transformation suppression 1
• Metastasin
• MTS1
• murine placental homolog)
• P9KA
• PEL98
• Placental calcium-binding protein
• Protein Mts1
• protein S100-A4
• S100 calcium binding protein A4
• S100 calcium-binding protein A4 (calcium protein, calvasculin, metastasin, murine placental homolog)
• S100 calcium-binding protein A4
• S100A4

Background

S100A4, also known as Metastasin, Mtsl and Calvasculin, is an 11 kDa member of the S100 (soluble in 100% saturated ammonium sulfate) family of proteins (1-5). The S100 family is further classified as a member of the EF-hand superfamily of Ca⁺⁺-binding proteins. These participate in both calcium-dependent and
calcium-independent protein-protein interactions. The hallmark of this superfamily is the EF-hand motif that consists of a Ca⁺⁺-binding site flanked by two alpha -helices (helix E and helix F) that were originally identified in a right-handed model of carp muscle calcium-binding protein (6). Human S100A4 is 101 amino acids (aa) in length (1, 2). It contains two EF hand domains, one between aa 12-47, and a second between aa 50-85. The first domain has a 14 aa cation-binding motif and binds Ca⁺⁺ with low affinity. The second Ca⁺⁺-binding motif is 12 aa in length and binds Ca⁺⁺ with high affinity. S100A4 has no classical signal sequence but is secreted from cells (3, 7). Human S100A4 shares 93% and 95% aa identity with mouse and canine S100A4, respectively. S100A4 reportedly exists as a dimer (8, 9, 10). Extracellular S100A4 is reported to induce MMP production, activate MMPs, promote neurite outgrowth and stimulate cardiomyocyte proliferation (4, 10, 11, 12, 13). Within the cell, dimers are likely the functional unit. Here, they are constitutive homo- or heterodimers (with S100A1) that interact with Ca⁺⁺, undergo a conformational change, and subsequently bind to cytoplasmic targets. Known targets include p53, myosin heavy chain II, F-actin and liprin beta 1 (4, 14). In general, it can be said that S100A4 blocks target phosphorylation and multimerization (4, 7, 14). S100A4 activity has been associated with cell transformation. It seems likely this is either coincidental, or a consequence, rather than a cause of transformation (3).

1. Engelkamp, D. et al. (1992) Biochemistry 31:10258.
2. Tomida, Y. et al. (1992) Biochem. Biophys. Res. Commun. 189:1310.
3. Garrett, S.C. et al. (2006) J. Biol. Chem. 281:677.
4. Santamaria-Kisiel, L. et al. (2006) Biochem. J. 396:201.
5. Donato, R. (2001) Int. J. Biochem. Mol. Biol. 33:637.
6. Kretsinger, R.H. and C.E. Nockolds (1973) J. Biol. Chem. 248:3313.
7. Helfman, D.M. et al. (2005) Br. J. Cancer 92:1955.
8. Burkitt, W.I. et al. (2003) Biochem. Soc. Trans. 31:985.
9. Vallaly, K.M. et al. (2002) Biochemistry 41:12670.
10. Novitskaya, V. et al. (2000) J. Biol. Chem. 275:41278.
11. Stary, M. et al. (2006) Biochem. Biophys. Res. Commun. 343:555.
12. Semov, A. et al. (2005) J. Biol. Chem. 280:20833.
13. Saleem, M. et al. (2006) Proc. Natl. Acad. Sci. USA 103:14825.
14. Kriajevska, M. et al. (2002) J. Biol. Chem. 277:5229.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for S100A4 Antibody (MAB4137)(1)

Publication using MAB4137

• Ji, S;Yu, H;Zhou, D;Fan, X;Duan, Y;Tan, Y;Lang, M;Shao, G; Cancer stem cell-derived CHI3L1 activates the MAF/CTLA4 signaling pathway to promote immune escape in triple-negative breast cancer Journal of translational medicine 2023-10-14 [PMID: 37838657] (Bioassay, Human)

Bioinformatics

• Gene Symbol: S100A4
• Uniprot:
  • P26447()