S100A16 Antibody - Azide and BSA Free

Western Blot: S100A16 Antibody [NBP2-99717] - Anti-S100A16 rabbit polyclonal antibody at 1:500 dilution. Lane A: PC3 Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 11 kDa. Observed band size: 11 kDa

Immunohistochemistry-Paraffin: S100A16 Antibody [NBP2-99717] - Immunochemical staining of human S100A16 in human brain with rabbit polyclonal antibody at 1:5000 dilution, formalin-fixed paraffin embedded sections.

Immunoprecipitation: S100A16 Antibody [NBP2-99717] - S100A16 was immunoprecipitated using: Lane A: 0.5 mg K562 Whole Cell Lysate0.5 ul anti-S100A16 rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose. Primary antibody: Anti-S100A16 rabbit polyclonal antibody, at 1:500 dilution. Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 11 kDa. Observed band size: 11 kDa

Immunohistochemistry-Paraffin: S100A16 Antibody [NBP2-99717] - Immunochemical staining of human S100A16 in human brain with NBP2-99717 at 1:5000 dilution, formalin-fixed paraffin embedded sections.

• Reactivity: Hu
• Applications: WB, ELISA, IHC, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: Azide and BSA Free

S100A16 Antibody - Azide and BSA Free Summary

• Description: This antibody can be stored at 2C to 8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.
• Immunogen: Produced in rabbits immunized with purified, recombinant Human S100A16 (Uniprot#: Q96FQ6; Met 1-Ser 103)
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: S100A16
• Purity: Antigen and protein A Affinity-purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:25000-1:50000
  • Immunohistochemistry-Paraffin 1:2000-1:10000
  • Immunoprecipitation 1-2 uL/mg of lysate
  • Western Blot 1:500-1:1000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 0.2 um filtered solution in PBS
• Preservative: No Preservative
• Purity: Antigen and protein A Affinity-purified

Alternate Names for S100A16 Antibody - Azide and BSA Free

• AAG13
• Aging-associated gene 13 protein
• aging-associated protein 13
• DT1P1A7
• MGC17528
• protein S100-A16
• Protein S100-F
• S100 calcium binding protein A16
• S100A16
• S100F
• S100FS100 calcium-binding protein A16

Background

S100A16 is a gene that codes for a calcium-binding protein that is 103 amino acids long, with a weight of approximately 12 kDa. Current research is being done on diseases and disorders linked to this gene including carcinoma. S100A16 has also been shown to have interactions with S100A14, UCHL5, GABARAPL1, CERS2, and ECM1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: S100A16