RXR alpha/NR2B1 Antibody

Western Blot: RXR alpha/NR2B1 Antibody [NB100-1466] - Western blot analysis of nuclear HeLa cell lysate (A) + peptide (B) and nuclear K562 cell lysate (C) + peptide (D). 35 ug protein in RIPA buffer. Antibody at 0.3 ug/mL. Detected by chemiluminescence.

Immunocytochemistry/Immunofluorescence: RXR alpha/NR2B1 Antibody [NB100-1466] - Immunofluorescence analysis of paraformaldehyde fixed MCF7 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing strong nuclear staining. Actin filaments were stained with phalloidin (red) and the nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).

Flow Cytometry: RXR alpha/NR2B1 Antibody [NB100-1466] - Flow cytometric analysis of paraformaldehyde fixed MCF7 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (1 ug/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ELISA, Flow, ICC/IF
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

RXR alpha/NR2B1 Antibody Summary

• Immunogen: Peptide with sequence CQVNSSLTSPTGRGSM corresponding to internal region according to NP_002948.1.
• Specificity: This antibody is expected to recognise an epitope corresponding to aa 14-28 of human RXR alpha protein and does not cross-react with either RXR beta or gamma.
• Predicted Species: Mouse (100%), Rat (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: RXRA
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 10 ug/mL
  • Immunocytochemistry/ Immunofluorescence 10 ug/mL
  • Peptide ELISA Detection limit 1:32000
  • Western Blot 0.3 - 1 ug/mL
• Application Notes: Western blot: Approx 55-60 kDa band observed in nuclear lysates of cell lines HeLa and K562 (calculated MW of 50.8 kDa according to NP_002948.1). This molecular weight is routinely observed by other sources and was successfully blocked by incubation with the immunizing peptide.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for RXR alpha/NR2B1 Antibody

• FLJ00280
• FLJ00318
• FLJ16020
• MGC102720
• NR2B1
• NR2B1FLJ16733
• Nuclear receptor subfamily 2 group B member 1
• retinoic acid receptor RXR-alpha
• Retinoid X receptor alpha
• retinoid X receptor, alpha
• RXR alpha
• RXRA

Background

Retinoid X receptors (RXRs) are members of the steroid/thyroid hormone receptor superfamily of nuclear receptor proteins which exert their effects by binding to specific DNA response elements, thus regulating gene expression in target cells. The RXR subfamily consists of at least three similar genes, RXR alpha, RXR beta and RXR gamma, all of which control transcription of target genes mediated by retinoids. RXR beta controls expression of many genes that respond to hormones and vitamins, including thyroid hormone, estrogen, retinoids and vitamin D. RXR beta controls a wide array of genes because of its ability to heterodimerize with other hormone receptors including the thyroid hormone receptor (THR), vitamin D receptor (VDR) and retinoic acid receptor (RAR).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for RXR alpha/NR2B1 Antibody (NB100-1466)(2)

Publications using NB100-1466

• Trumper V, von Knethen A, PreuB A et al. Flow cytometry-based FRET identifies binding intensities in PPAR gamma 1 protein-protein interactions in living cells Theranostics [PMID: 31534496] (WB, Human)
• Claudel T, Leibowitz MD, Fievet C et al. Reduction of atherosclerosis in apolipoprotein E knockout mice by activation of the retinoid X receptor. Proc Natl Acad Sci U S A 2001-02-27 [PMID: 11226287]

Bioinformatics

• Gene Symbol: RXRA
• Entrez:
  • 6256(Human)
• Uniprot:
  • NP_002948.(Human)