RUVBL1 Antibody

Western Blot: RUVBL1 Antibody [NBP2-27147] - analysis of Rvb1 in human testis lysate in the 1) absence and 2) presence of immunizing peptide, 3) mouse testis and 4) rat testis lysate using this antibody. I goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.

Immunohistochemistry-Paraffin: RUVBL1 Antibody [NBP2-27147] - Immunohistochemical analysis of Rvb1 in formalin-fixed, paraffin-embedded mouse testis tissue using NBP2-27147 at 5 ug/ml.

• Reactivity: Hu, Mu, Rt
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

RUVBL1 Antibody Summary

• Description: Novus Biologicals Rabbit RUVBL1 Antibody (NBP2-27147) is a polyclonal antibody validated for use in IHC and WB. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: A portion of amino acids 400-454 of human Rvb1 was used as the immunogen.
• Predicted Species: Mouse (100%), Rat (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: RUVBL1
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Western Blot 1:100-1:2000

Reactivity Notes

The amino acid sequence used as immunogen is 100% homologous in mouse and rat.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS and 0.05% BSA
• Preservative: 0.05% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Protein A purified

Alternate Names for RUVBL1 Antibody

• 49 kDa TATA box-binding protein-interacting protein
• 54 kDa erythrocyte cytosolic protein
• EC 3.6.1
• EC 3.6.4.12,49 kDa TBP-interacting protein
• ECP54
• INO80 complex subunit H
• INO80HTIP49A
• NMP 238
• NMP238ECP-54
• Nuclear matrix protein 238
• Pontin 52
• PONTIN
• Pontin52
• RuvB (E coli homolog)-like 1
• RuvB-like 1 (E. coli)
• ruvB-like 1
• RVB1
• TATA binding protein interacting protein 49 kDa
• TIH1
• TIP49a
• TIP49TAP54-alpha
• TIP60-associated protein 54-alpha

Background

RuvB-like1 (Rvb1) is a member of the AAA+ superfamily, and has both single-stranded DNA-stimulated ATPase and ATP-dependent DNA helicase (3' to 5') activity. AAA+ stands for "ATPases associated with diverse cellular activities and AAA+ family members share similarity in sequence and in structure. As a family, AAA+ proteins are involved in a large number of cellular functions in which energy extracted from ATP hydrolysis is used in molecular remodeling events (reviewed in Snider et al, 2008). Functional diversity and specificity of family members is obtained through the addition of structural motifs and modifications to a core AAA+ module as well as by the association of partner proteins and cofactors. Rvb1 and the closely related Rvb2 protein are both involved in chromatin remodeling, transcription, small nucleolar RNA maturation, cellular transformation, apoptosis, and mitosis (reviewed in Cheung et al, 2010 and Huen et al, 2010). Rvbs are capable of playing roles in such diverse cellular processes because they are components of a number of different macromolecular assemblies. For example, Rvbs are componentsof large chromatin-remodeling complexes including INO80, p400, and SWR1 and of chromatin-modifying enzymes such as the TIP60 complex. Rvbs are also found in RNA polymerase II and small nucleolar ribonucleoprotein complexes where they play roles in mediating transcription and nucelolar localization, respectively. Rvbs have roles in both transformation and apoptosis through their interaction with c-MYC, and in mitosis through their association with the mitotic spindle and centrosomes. Rvbs have been implicated in human pathologies, including carcinogenesis. For example Rvb1 overexpresssion has been reported in colon carcinoma, and Rvb1 SUMOylation has been shown to increase the proliferation and growth of prostate cancer cells. Rvb1 may also play a role in autoimmunity as high levels of RUVB1 autoantibodies have been detected in the sera of patients with autoimmune diseases including polymyositis/dermatomyosistic and autoimmune hepatitis. The extensive involvement of Rvbs in DNA-associated processes, including their interaction with a wide range of transcription factors, suggests that Rvb dysregulation may be involved in a number of other pathological processes yet to be elucidated. The IMG-6595A antibody recognizes Rvb1. Rvb1 is also known as Rubvl1, ECP54, TIP49, NMP238, TIP49A, LOC8607, RuvB-like 1, RuvB (E coli homolog)-like 1, and TATA binding protein interacting protein 49 kDa.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: RUVBL1
• Uniprot:
  • Q9Y265(Human)