| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-84914-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-84913-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-59456-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-22244-50ul | |||
![Immunocytochemistry/Immunofluorescence: RUVBL1 Antibody [NBP2-20245] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: RUVBL1 protein stained by RUVBL1 antibody diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 um.](http://images.novusbio.com/fullsize/RUVBL1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-20245-img0012.jpg "Immunocytochemistry/Immunofluorescence: RUVBL1 Antibody [NBP2-20245] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: RUVBL1 protein stained by RUVBL1 antibody diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 um.")
![Immunohistochemistry-Paraffin: RUVBL1 Antibody [NBP2-20245] - Mouse testis. RUVBL1 antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/RUVBL1-Antibody-Immunohistochemistry-Paraffin-NBP2-20245-img0010.jpg)
![Immunohistochemistry-Paraffin: RUVBL1 Antibody [NBP2-20245] - Human colon carcinoma, using RUVBL1antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/RUVBL1-Antibody-Immunohistochemistry-Paraffin-NBP2-20245-img0002.jpg)
![Immunohistochemistry-Paraffin: RUVBL1 Antibody [NBP2-20245] - Rat heart. RUVBL1 antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/RUVBL1-Antibody-Immunohistochemistry-Paraffin-NBP2-20245-img0007.jpg)
![Immunohistochemistry-Paraffin: RUVBL1 Antibody [NBP2-20245] - Mouse colon. RUVBL1 antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/RUVBL1-Antibody-Immunohistochemistry-Paraffin-NBP2-20245-img0008.jpg)
![Immunohistochemistry-Paraffin: RUVBL1 Antibody [NBP2-20245] - Rat testis. RUVBL1 antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/RUVBL1-Antibody-Immunohistochemistry-Paraffin-NBP2-20245-img0009.jpg)
![Immunoprecipitation: RUVBL1 Antibody [NBP2-20245] - Immunoprecipitation of RUVBL protein from Jurkat whole cell extracts using 5 ug of RUVBL1 antibody. Western blot analysis was performed using RUVBL1 antibody. EasyBlot anti-Rabbit IgG was used as a secondary reagent.](http://images.novusbio.com/fullsize/RUVBL1-Antibody-Immunoprecipitation-NBP2-20245-img0006.jpg)
![Immunocytochemistry/ Immunofluorescence: RUVBL1 Antibody [NBP2-20245] - Endogenous RUVBL1 & RUVBL2 colocalize with HA-NP. HeLa cells were transfected with vector control, HA-NP, or HA-VP35. Twenty-four h later, the cells were fixed & processed for immunofluorescence detection of endogenous RUVBL1 or RUVBL2 in the presence of vector control, HA-NP, or HA-VP35. Representative images of (A) endogenous RUVBL1 localization pattern with control vector (top panels), HA-NP (middle panels), or HA-VP35 (bottom panels) & (B) endogenous RUVBL2 localization pattern with control vector (top panels), HA-NP (middle panels), or HA-VP35 (bottom panels) are shown. HA-NP or HA-VP35 (green), RUVBL1/2 (red), & Hoechst 33342 nuclear stain (blue) were visualized by confocal microscopy. Scale bars = 20 µM. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31018511), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp2-20245_rabbit-polyclonal-ruvbl1-antibody-31020241534318.jpg)
![Western Blot: RUVBL1 Antibody [NBP2-20245] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with RUVBL1 antibody (NBP2-20245) diluted at 1:1000.](http://images.novusbio.com/fullsize/nbp2-20245_rabbit-polyclonal-ruvbl1-antibody-91020242063352.jpg)
![Western Blot: RUVBL1 Antibody [NBP1-84914] - Analysis in MCF-7 cells transfected with control siRNA, target specific siRNA probe #1, using Anti-RUVBL1 antibody. Remaining relative intensity is presented. Loading control: Anti-PPIB.](http://images.novusbio.com/fullsize/RUVBL1-Antibody-Western-Blot-NBP1-84914-img0010.jpg "<b>Genetic Strategies Validation. </b>Western Blot: RUVBL1 Antibody [NBP1-84914] - Analysis in MCF-7 cells transfected with control siRNA, target specific siRNA probe #1, using Anti-RUVBL1 antibody. Remaining relative intensity is presented. Loading control: Anti-PPIB.")
![<b>Genetic Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: RUVBL1 Antibody [NBP1-84914] - Staining of human cell line A-431 shows localization to nucleoplasm & cytosol. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/RUVBL1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-84914-img0008.jpg)
![Immunohistochemistry-Paraffin: RUVBL1 Antibody [NBP1-84914] - Staining of human fallopian tube using Anti-RUVBL1 antibody NBP1-84914.](http://images.novusbio.com/fullsize/RUVBL1-Antibody-Immunohistochemistry-Paraffin-NBP1-84914-img0018.jpg)
![Immunohistochemistry-Paraffin: RUVBL1 Antibody [NBP1-84914] - Staining of human fallopian tube shows high expression.](http://images.novusbio.com/fullsize/RUVBL1-Antibody-Immunohistochemistry-Paraffin-NBP1-84914-img0011.jpg)
![Immunohistochemistry-Paraffin: RUVBL1 Antibody [NBP1-84914] - Staining of human skeletal muscle shows low expression as expected.](http://images.novusbio.com/fullsize/RUVBL1-Antibody-Immunohistochemistry-Paraffin-NBP1-84914-img0012.jpg)
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: RUVBL1 Antibody [NBP1-84914] - Staining in human fallopian tube and skeletal muscle tissues using anti-RUVBL1 antibody. Corresponding RUVBL1 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/RUVBL1-Antibody-Immunohistochemistry-Paraffin-NBP1-84914-img0013.jpg)
![<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: RUVBL1 Antibody [NBP1-84914] - Staining of human fallopian tube, kidney, lymph node and testis using Anti-RUVBL1 antibody NBP1-84914 (A) shows similar protein distribution across tissues to independent antibody NBP1-84913 (B).](http://images.novusbio.com/fullsize/RUVBL1-Antibody-Immunohistochemistry-Paraffin-NBP1-84914-img0014.jpg)
![Immunohistochemistry-Paraffin: RUVBL1 Antibody [NBP1-84914] - Staining of human kidney.](http://images.novusbio.com/fullsize/RUVBL1-Antibody-Immunohistochemistry-Paraffin-NBP1-84914-img0015.jpg)
![Immunohistochemistry-Paraffin: RUVBL1 Antibody [NBP1-84914] - Staining of human lymph node.](http://images.novusbio.com/fullsize/RUVBL1-Antibody-Immunohistochemistry-Paraffin-NBP1-84914-img0016.jpg)
![Immunohistochemistry-Paraffin: RUVBL1 Antibody [NBP1-84914] - Staining of human testis.](http://images.novusbio.com/fullsize/RUVBL1-Antibody-Immunohistochemistry-Paraffin-NBP1-84914-img0017.jpg)
![Immunohistochemistry-Paraffin: RUVBL1 Antibody [NBP1-84913] - Staining of human fallopian tube using Anti-RUVBL1 antibody NBP1-84913.](http://images.novusbio.com/fullsize/RUVBL1-Antibody-Immunohistochemistry-Paraffin-NBP1-84913-img0013.jpg "Immunohistochemistry-Paraffin: RUVBL1 Antibody [NBP1-84913] - Staining of human fallopian tube using Anti-RUVBL1 antibody NBP1-84913.")
![Immunohistochemistry-Paraffin: RUVBL1 Antibody [NBP1-84913] - Staining of human testis shows moderate cytoplasmic positivity in cells in seminiferous ducts.](http://images.novusbio.com/fullsize/RUVBL1-Antibody-Immunohistochemistry-Paraffin-NBP1-84913-img0005.jpg)
![Immunohistochemistry-Paraffin: RUVBL1 Antibody [NBP1-84913] - Staining of human fallopian tube shows high expression.](http://images.novusbio.com/fullsize/RUVBL1-Antibody-Immunohistochemistry-Paraffin-NBP1-84913-img0006.jpg)
![Immunohistochemistry-Paraffin: RUVBL1 Antibody [NBP1-84913] - Staining of human skeletal muscle shows low expression as expected.](http://images.novusbio.com/fullsize/RUVBL1-Antibody-Immunohistochemistry-Paraffin-NBP1-84913-img0007.jpg)
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: RUVBL1 Antibody [NBP1-84913] - Staining in human fallopian tube and skeletal muscle tissues using anti-RUVBL1 antibody. Corresponding RUVBL1 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/RUVBL1-Antibody-Immunohistochemistry-Paraffin-NBP1-84913-img0008.jpg)
![<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: RUVBL1 Antibody [NBP1-84913] - Staining of human fallopian tube, kidney, lymph node and testis using Anti-RUVBL1 antibody NBP1-84913 (A) shows similar protein distribution across tissues to independent antibody NBP1-84914 (B).](http://images.novusbio.com/fullsize/RUVBL1-Antibody-Immunohistochemistry-Paraffin-NBP1-84913-img0009.jpg)
![Immunohistochemistry-Paraffin: RUVBL1 Antibody [NBP1-84913] - Staining of human testis.](http://images.novusbio.com/fullsize/RUVBL1-Antibody-Immunohistochemistry-Paraffin-NBP1-84913-img0010.jpg)
![Immunohistochemistry-Paraffin: RUVBL1 Antibody [NBP1-84913] - Staining of human kidney.](http://images.novusbio.com/fullsize/RUVBL1-Antibody-Immunohistochemistry-Paraffin-NBP1-84913-img0011.jpg)
![Immunohistochemistry-Paraffin: RUVBL1 Antibody [NBP1-84913] - Staining of human lymph node.](http://images.novusbio.com/fullsize/RUVBL1-Antibody-Immunohistochemistry-Paraffin-NBP1-84913-img0012.jpg)
![Western Blot: RUVBL1 Antibody (7Q2U8) [NBP3-16583] - Western blot analysis of extracts of various cell lines, using RUVBL1 Rabbit mAb (NBP3-16583) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.](http://images.novusbio.com/fullsize/RUVBL1-Antibody-7Q2U8-Western-Blot-NBP3-16583-img0002.jpg "Western Blot: RUVBL1 Antibody (7Q2U8) [NBP3-16583] - Western blot analysis of extracts of various cell lines, using RUVBL1 Rabbit mAb (NBP3-16583) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.")
![Immunohistochemistry-Paraffin: RUVBL1 Antibody (7Q2U8) [NBP3-16583] - Immunohistochemistry of paraffin-embedded mouse testis using RUVBL1 Rabbit mAb (NBP3-16583) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/RUVBL1-Antibody-7Q2U8-Immunohistochemistry-Paraffin-NBP3-16583-img0001.jpg)
![Chromatin Immunoprecipitation: RUVBL1 Antibody (7Q2U8) [NBP3-16583] -Analysis of extracts of HepG2 cells, using RUVBL1/TIP49A/PONTIN Rabbit mAb antibody and rabbit IgG.The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.](http://images.novusbio.com/fullsize/nbp3-16583_rabbit-ruvbl1-mab-7q2u8-chromatin-immunoprecipitation-78202411528..jpg)
![Chromatin Immunoprecipitation: RUVBL1 Antibody (7Q2U8) [NBP3-16583] - Chromatin immunoprecipitation analysis of extracts of HepG2 cells, using RUVBL1 Rabbit mAb antibody and rabbit IgG.The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.](http://images.novusbio.com/fullsize/nbp3-16583_rabbit-ruvbl1-mab-7q2u8-522025126537.jpg)
![Chromatin Immunoprecipitation: RUVBL1 Antibody (7Q2U8) [RUVBL1] - Chromatin immunoprecipitation analysis of extracts of HepG2 cells, using RUVBL1 Rabbit mAb antibody and rabbit IgG.The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.](http://images.novusbio.com/fullsize/nbp3-16583_rabbit-ruvbl1-mab-7q2u8-52202517162323.jpg)
![Western Blot: RUVBL1 Antibody [NBP2-27147] - analysis of Rvb1 in human testis lysate in the 1) absence and 2) presence of immunizing peptide, 3) mouse testis and 4) rat testis lysate using this antibody. I goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.](http://images.novusbio.com/fullsize/RUVBL1-Antibody-Western-Blot-NBP2-27147-img0002.jpg "Western Blot: RUVBL1 Antibody [NBP2-27147] - analysis of Rvb1 in human testis lysate in the 1) absence and 2) presence of immunizing peptide, 3) mouse testis and 4) rat testis lysate using this antibody. I goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.")
![Immunohistochemistry-Paraffin: RUVBL1 Antibody [NBP2-27147] - Immunohistochemical analysis of Rvb1 in formalin-fixed, paraffin-embedded mouse testis tissue using NBP2-27147 at 5 ug/ml.](http://images.novusbio.com/fullsize/RUVBL1-Antibody-Immunohistochemistry-Paraffin-NBP2-27147-img0003.jpg)
![Western Blot: RUVBL1 Antibody (3G4-1F8) [H00008607-M01] - RUVBL1 monoclonal antibody (M01), clone 3G4-1F8. Analysis of RUVBL1 expression in human pancreas.](http://images.novusbio.com/fullsize/RUVBL1-Antibody-3G4-1F8-Western-Blot-H00008607-M01-img0007.jpg "Western Blot: RUVBL1 Antibody (3G4-1F8) [H00008607-M01] - RUVBL1 monoclonal antibody (M01), clone 3G4-1F8. Analysis of RUVBL1 expression in human pancreas.")
![Immunocytochemistry/Immunofluorescence: RUVBL1 Antibody (3G4-1F8) [H00008607-M01] - Analysis of monoclonal antibody to RUVBL1 on HeLa cell . Antibody concentration 10 ug/ml.](http://images.novusbio.com/fullsize/RUVBL1-Antibody-3G4-1F8-Immunocytochemistry-Immunofluorescence-H00008607-M01-img0005.jpg)
![Western Blot: RUVBL1 Antibody (3G4-1F8) [H00008607-M01] - Analysis of RUVBL1 expression in transfected 293T cell line by RUVBL1 monoclonal antibody (M01), clone 3G4-1F8.Lane 1: RUVBL1 transfected lysate (Predicted MW: 50.2 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/RUVBL1-Antibody-3G4-1F8-Western-Blot-H00008607-M01-img0008.jpg)
![Proximity Ligation Assay: RUVBL1 Antibody (3G4-1F8) [H00008607-M01] - Analysis of protein-protein interactions between MAP3K7 and RUVBL1. HeLa cells were stained with anti-MAP3K7 rabbit purified polyclonal 1:1200 and anti-RUVBL1 mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/RUVBL1-Antibody-3G4-1F8-Proximity-Ligation-Assay-H00008607-M01-img0003.jpg)
![ELISA: RUVBL1 Antibody (3G4-1F8) [H00008607-M01] - Detection limit for recombinant GST tagged RUVBL1 is approximately 0.3ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/RUVBL1-Antibody-3G4-1F8-ELISA-H00008607-M01-img0009.jpg)
![Western Blot: RUVBL1 Antibody (1D6) [NBP2-59456] - Lane 1: Raji cell lysates](http://images.novusbio.com/fullsize/RUVBL1-Antibody-1D6-Western-Blot-NBP2-59456-img0001.jpg "Western Blot: RUVBL1 Antibody (1D6) [NBP2-59456] - Lane 1: Raji cell lysates")
![Immunocytochemistry/Immunofluorescence: RUVBL1 Antibody (1D6) [NBP2-59456] - Analysis of RuVBL in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human RuVBL antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).](http://images.novusbio.com/fullsize/RUVBL1-Antibody-1D6-Immunocytochemistry-Immunofluorescence-NBP2-59456-img0002.jpg)
![Western Blot: RUVBL1 Antibody [NBP2-20244] - Sample (30 ug of whole cell lysate) A: A549 B: H1299 C: HCT116 D: MCF-7 10% SDS PAGE gel, diluted at 1:1000.](http://images.novusbio.com/fullsize/RUVBL1-Antibody-Western-Blot-NBP2-20244-img0001.jpg "Western Blot: RUVBL1 Antibody [NBP2-20244] - Sample (30 ug of whole cell lysate) A: A549 B: H1299 C: HCT116 D: MCF-7 10% SDS PAGE gel, diluted at 1:1000.")
![<b>Genetic Strategies Validation. </b>Western Blot: RUVBL1 Antibody [NBP2-20244] - Non-transfected (–) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with RUVBL1 antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-20244_rabbit-polyclonal-ruvbl1-antibody-1610202419405763.jpg)
![Immunoprecipitation:RUVBL1 AntibodyNBP3-29820] -Detection of human RUVBL1 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-RUVBL1 antibody NBP3-29820 (lot NBP3-29820-1) used for IP at 6 µg per reaction. RUVBL1 was also immunoprecipitated by rabbit anti-RUVBL1.. For blotting immunoprecipitated RUVBL1, NBP3-29820 was used at 0.4 µg/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.](http://images.novusbio.com/fullsize/nbp3-29820_rabbit-ruvbl1-pab-301220242041573.jpg "Immunoprecipitation:RUVBL1 AntibodyNBP3-29820] -Detection of human RUVBL1 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-RUVBL1 antibody NBP3-29820 (lot NBP3-29820-1) used for IP at 6 µg per reaction. RUVBL1 was also immunoprecipitated by rabbit anti-RUVBL1.. For blotting immunoprecipitated RUVBL1, NBP3-29820 was used at 0.4 µg/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.")
![Western Blot: RUVBL1 Antibody (SR1895) [NBP3-22244] - Western blot analysis using NBP3-22244 in K562 cell lysate.](http://images.novusbio.com/fullsize/nbp3-22244_rabbit-ruvbl1-mab-sr1895-5720231624723.jpg "Western Blot: RUVBL1 Antibody (SR1895) [NBP3-22244] - Western blot analysis using NBP3-22244 in K562 cell lysate.")
![SDS-Page: Recombinant Human RUVBL1 Protein [NBP1-50845] - 15 % SDS-PAGE (3ug)](http://images.novusbio.com/fullsize/Recombinant-Human-RUVBL1-Protein-SDS-Page-NBP1-50845-img0001.jpg "SDS-Page: Recombinant Human RUVBL1 Protein [NBP1-50845] - 15 % SDS-PAGE (3ug)")
![Western Blot: RUVBL1 Overexpression Lysate (Adult Normal) [NBL1-15631] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for RUVBL1.](http://images.novusbio.com/fullsize/RUVBL1-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-15631-img0002.jpg "Western Blot: RUVBL1 Overexpression Lysate (Adult Normal) [NBL1-15631] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for RUVBL1.")