RSK1 [p Thr359, p Ser363] Antibody (S01-5B1)

Western Blot: RSK1 [p Thr359, p Ser363] Antibody (S01-5B1) [NBP3-20023] - Western blot detection of RSK1 in C6 cell lysates using NBP3-20023 (1:1000 diluted). Predicted band size: 83kDa. Observed band size: 90kDa.

• Reactivity: Hu, Mu, Rt
• Applications: WB
• Clone: S01-5B1
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 0.3 mg/ml

RSK1 [p Thr359, p Ser363] Antibody (S01-5B1) Summary

• Description: Novus Biologicals Rabbit RSK1 [p Thr359, p Ser363] Antibody (S01-5B1) (NBP3-20023) is a recombinant monoclonal antibody validated for use in WB. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: A synthetic phosphopeptide corresponding to residues surrounding Thr359/Ser363 of human RSK1 (Uniprot # Q15418)
• Modification: p Thr359, p Ser363
• Source: HEK293
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: RPS6KA1
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1/1000
• Theoretical MW: 90 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.05% BSA
• Preservative: 0.01% Sodium Azide
• Concentration: 0.3 mg/ml
• Purity: Affinity purified

Alternate Names for RSK1 [p Thr359, p Ser363] Antibody (S01-5B1)

• 90kD, polypeptide 1)
• EC 2.7.11
• EC 2.7.11.1,90 kDa ribosomal protein S6 kinase 1
• HU-1
• MAP kinase-activated protein kinase 1a
• MAPK-activated protein kinase 1a
• MAPKAP kinase 1a
• MAPKAPK-1a
• MAPKAPL1A
• p90RSK1
• p90S6K
• ribosomal protein S6 kinase alpha 1
• ribosomal protein S6 kinase alpha-1
• ribosomal protein S6 kinase, 90kD, polypeptide 1
• ribosomal protein S6 kinase, 90kDa, polypeptide 1
• Ribosomal S6 kinase 1
• RPS6KA1
• RSK
• RSK1
• RSK-1
• RSK1p90-RSK 1
• S6K-alpha 1
• S6K-alpha-1

Background

Rsk-1 (p90-Rsk-1) is a member of a family of 90 kDa ribosomal protein S6 kinases, which includes Rsk-1, Rsk-2 and Rsk-3. These are broadly expressed serine/threonine protein kinases (1) activated in response to mitogenic stimuli, including extracellular signal-regulated protein kinases Erk1 and Erk2 (2). p90-Rsk-1 is activated by MAPK in vitro and in vivo via phosphorylation (3). Active Rsks appear to play a major role in transcriptional regulation by translocating to the nucleus and phosphorylating c-Fos and CREB (2,4).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: RPS6KA1