RRP1 Antibody - BSA Free

Western Blot: RRP1 Antibody [NBP1-85338] - Analysis in human cell line K562.

Immunocytochemistry/Immunofluorescence: RRP1 Antibody [NBP1-85338] - Staining of human cell line A-431 shows localization to nucleoli. Antibody staining is shown in green.

Immunohistochemistry-Paraffin: RRP1 Antibody [NBP1-85338] - Staining of human skeletal muscle shows no nucleoli positivity in monocytes.

Immunohistochemistry-Paraffin: RRP1 Antibody [NBP1-85338] - Staining of human skin shows weak positivity in nucleoli in squamous epithelial cells.

Immunohistochemistry-Paraffin: RRP1 Antibody [NBP1-85338] - Staining of human cerebral cortex shows weak positivity in nucleoli in neurons.

Immunohistochemistry-Paraffin: RRP1 Antibody [NBP1-85338] - Staining of human duodenum shows moderate nuclear positivity in glandular cells.

• Reactivity: Hu
• Applications: WB, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

RRP1 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit RRP1 Antibody - BSA Free (NBP1-85338) is a polyclonal antibody validated for use in IHC, WB and ICC/IF. Anti-RRP1 Antibody: Cited in 3 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: This antibody was developed against Recombinant Protein corresponding to amino acids: NLKFIDPFCRIAARTKDSLVLNNITRGIFETIVEQAPLAIEDLLNELDTQDEEVASDSDESSEGGERGDALSQKRSEKPPA
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: RRP1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:200 - 1:500
  • Immunohistochemistry-Paraffin 1:200-1:500
  • Western Blot 0.04-0.4 ug/ml
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
• Control Peptide:

  RRP1 Protein (NBP1-85338PEP)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Immunogen affinity purified

Alternate Names for RRP1 Antibody - BSA Free

• D21S2056ENop52
• NNP1
• NNP-1Nnp1 homolog, nucleolar protein
• NOP52protein NNP-1
• Novel nuclear protein 1
• Nucleolar protein Nop52
• ribosomal RNA processing 1 homolog (S. cerevisiae)
• RRP1Aribosomal RNA processing protein 1 homolog A
• RRP1-like protein

Background

RRP1 is encoded by this gene is the putative homolog of the yeast ribosomal RNA processing protein RRP1. The encoded protein is involved in the late stages of nucleologenesis at the end of mitosis, and may be required for the generation of 28S rRNA.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for RRP1 Antibody (NBP1-85338)(3)

Publications using NBP1-85338

• Liskovykh M, Petrov N, Noskov V et al. Actively transcribed rDNA and distal junction (DJ) sequence determine association of NORs with nucleoli Research Square 2022-08-04 [PMID: 37043028] (ICC/IF, Human)
• van Sluis M, van Vuuren C, McStay B. The Relationship Between Human Nucleolar Organizer Regions and Nucleoli, Probed by 3D-ImmunoFISH. Methods Mol. Biol. 2016-01-01 [PMID: 27576706]
• Uhlen M, Oksvold P, Algenas C et al. Antibody-based Protein Profiling of the Human Chromosome 21. Mol Cell Proteomics 2012-03-01 [PMID: 22042635]

Bioinformatics

• Gene Symbol: RRP1