RRP1B Antibody - BSA Free

Western Blot: RRP1B Antibody [NBP1-81533] - Analysis in U2OS cells transfected with control siRNA, target specific siRNA probe #1 and #2. Remaining relative intensity is presented.

Immunocytochemistry/Immunofluorescence: RRP1B Antibody [NBP1-81533] - Immunofluorescent staining of human cell line A-431 shows localization to nucleoli.

Immunohistochemistry-Paraffin: RRP1B Antibody [NBP1-81533] - Staining of human tonsil shows moderate to strong nuclear positivity in non-germinal center cells.

Immunohistochemistry-Paraffin: RRP1B Antibody [NBP1-81533] - Staining of human cerebellum shows moderate to strong nuclear positivity in cells in molecular layer.

Immunohistochemistry-Paraffin: RRP1B Antibody [NBP1-81533] - Staining of human skin shows moderate to strong nuclear positivity in keratinocytes.

Immunohistochemistry-Paraffin: RRP1B Antibody [NBP1-81533] - Staining of human testis shows moderate to strong nuclear positivity in cells in seminiferous ducts.

• Reactivity: Hu
• Applications: WB, ICC/IF, IHC, Mycoplasma
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

RRP1B Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit RRP1B Antibody - BSA Free (NBP1-81533) is a polyclonal antibody validated for use in IHC, WB and ICC/IF. Anti-RRP1B Antibody: Cited in 2 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: This antibody was developed against Recombinant Protein corresponding to amino acids: SISQLSFAEDISADEDDQILSQGKHKKKGNKLLEKTNLEKEKGSRVFCVEEEDSESSLQKRRRKKKKKHHLQPENPGPGGAAPSLEQNRGREPEASGLKALKARVAEPGAEATSSTG
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: RRP1B
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:500 - 1:1000
  • Immunohistochemistry-Paraffin 1:500 - 1:1000
  • Knockdown Validated
  • Western Blot 0.04-0.4 ug/ml
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
• Control Peptide:

  RRP1B Protein (NBP1-81533PEP)

Reactivity Notes

Reactivity reported in scientific literature (PMID: 23604122)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Immunogen affinity purified

Alternate Names for RRP1B Antibody - BSA Free

• Nnp1
• ribosomal RNA processing 1 homolog B (S. cerevisiae)
• RRP1

Background

The RRP1B gene enocdes a ribosomal RNA processing protein 1 homolog B that in isoform 1 is 758 amino acids and 84kDA and in isoform 2 is 740 amino acids long at 82 kDA. RRP1B is characterized by its interactions with genes ESR2, LSM1, LUC7L2, LUC7L3, and C7orf55-LUC7L2. It has been researched regarding its role in various diseases such as breast cancer and cancer progression in terms of metastasis efficiency.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for RRP1B Antibody (NBP1-81533)(2)

Publications using NBP1-81533

• Alsarraj J, Faraji F, Geiger TR et al. BRD4 Short Isoform Interacts with RRP1B, SIPA1 and Components of the LINC Complex at the Inner Face of the Nuclear Membrane. PLoS One 2013-01-01 [PMID: 24260471]
• Lee M, Dworkin AM, Gildea D et al. RRP1B is a Metastasis Modifier That Regulates the Expression of Alternative mRNA Isoforms Through Interactions with SRSF1. Oncogene 2014-04-03 [PMID: 23604122]

Bioinformatics

• Gene Symbol: RRP1B