RRBP1 Antibody (5610) - Azide and BSA Free

Western Blot: RRBP1 Antibody (5610) [NBP2-43600] - Non-transfected (-) and transfected (+) HepG2 whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with Ribosome binding protein 1 antibody [5610].

Immunocytochemistry/Immunofluorescence: RRBP1 Antibody (5610) [NBP2-43600] - Analysis of Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Ribosome binding protein 1 protein stained by Ribosome binding protein 1 antibody [5610] diluted at 1:2000. Blue: Hoechst 33342 staining.

Ribosome binding protein 1 antibody [GT5610] detects Ribosome binding protein 1 protein at endoplasmic reticulum by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: Ribosome binding protein 1 protein stained by Ribosome binding protein 1 antibody [GT5610] (NBP2-43600) diluted at 1:2000.Blue: Hoechst 33342 staining.Scale bar = 10 um.

• Reactivity: Hu
• Applications: WB, Func, ICC/IF
• Clone: 5610
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

RRBP1 Antibody (5610) - Azide and BSA Free Summary

• Description: Novus Biologicals Mouse RRBP1 Antibody (5610) - Azide and BSA Free (NBP2-43600) is a monoclonal antibody validated for use in WB and ICC/IF. Anti-RRBP1 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Recombinant protein encompassing a sequence within the C-terminus region of human RRBP1. The exact sequence is proprietary.
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: RRBP1
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100-1:2000
  • Proximity Ligation Assay Reported in scientific literature (PMID:35907632)
  • Western Blot 1:500-1:3000
• Theoretical MW: 152 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Mouse (81%), Dog (83%).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: No Preservative
• Purity: Protein G purified

Alternate Names for RRBP1 Antibody (5610) - Azide and BSA Free

• 180 kDa ribosome receptor homolog
• DKFZp586A1420
• ES/130
• ES/130-related protein
• ES130
• FLJ36146
• hES
• KIAA1398
• MGC157720
• MGC157721
• ribosome binding protein 1 (dog 180kD homolog)
• ribosome binding protein 1 homolog 180kDa (dog)
• Ribosome receptor protein
• ribosome-binding protein 1
• RRp

Background

Analysis of cDNA clones indicates that ribosome binding protein 1 may exist in different forms due to removal of tandem repeats, or partial intraexonic splicing of RRBP1. The form presented here is lacking the canine p180 ribosome-binding domain, NQGKKAEGAQ, which is tandemly repeated close to the N-terminus in other forms that haven't been fully characterized. RRBP1 has been excluded as a candidate gene in the cause of Alagille syndrome. Alternate splicing results in multiple transcript variants. (provided by RefSeq)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for RRBP1 Antibody (NBP2-43600)(1)

Publication using NBP2-43600

• Pourshafie N, Masati E, Lopez A et al. Altered SYNJ2BP-mediated mitochondrial-ER contacts in motor neuron disease Neurobiology of disease 2022-07-27 [PMID: 35907632] (ICC/IF, PLA, Human)

Bioinformatics

• Gene Symbol: RRBP1