RRAGA Antibody - BSA Free

Western Blot: RRAGA Antibody [NBP3-13446] - Whole cell extract (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with RRAGA antibody (NBP3-13446) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.

Immunocytochemistry/ Immunofluorescence: RRAGA Antibody [NBP3-13446] - RRAGA antibody detects RRAGA protein at cytoplasm and nucleus by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: RRAGA stained by RRAGA antibody (NBP3-13446) diluted at 1:500.Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] diluted at 1:1000.Scale bar= 10um.

Immunohistochemistry-Paraffin: RRAGA Antibody [NBP3-13446] - RRAGA antibody detects RRAGA protein at cytoplasm and nucleus by immunohistochemical analysis.Sample: Paraffin-embedded rat brain.RRAGA stained by RRAGA antibody (NBP3-13446) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Immunohistochemistry-Paraffin: RRAGA Antibody [NBP3-13446] - RRAGA antibody detects RRAGA protein at cytoplasm and nucleus by immunohistochemical analysis.Sample: Paraffin-embedded mouse brain.RRAGA stained by RRAGA antibody (NBP3-13446) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

• Reactivity: Hu
• Applications: WB
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

RRAGA Antibody - BSA Free Summary

• Description: Centrifuge briefly prior to opening.
• Immunogen: Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human RRAGA. The exact sequence is proprietary.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: RRAGA
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Western Blot

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS, 20% Glycerol
• Preservative: 0.025% Proclin 300
• Purity: Protein A purified

Alternate Names for RRAGA Antibody - BSA Free

• Adenovirus E3 14.7 kDa-interacting protein 1
• FIP1
• FIP-1adenovirus E3-14.7K interacting protein 1
• Rag A
• RagA
• RAGAras-related GTP-binding protein A
• Ras-related GTP binding A

Background

RRAGA is involved in the RCC1/Ran-GTPase pathway. May play a direct role in a TNF-alpha signaling pathway leading to induction of cell death. May alternatively act as a cellular target for adenovirus E3-14.7K, an inhibitor of TNF-alpha functions, thereby affecting

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: RRAGA