RPS3A Antibody - BSA Free

Western Blot: RPS3A Antibody [NBP2-94503] - Analysis of 200ug extracts of MCF7 cells using RPS3A at a dilition of 1:500.

Immunocytochemistry/Immunofluorescence: RPS3A Antibody [NBP2-94503] - Analysis of MCF-7 cells using RPS3A . Blue: DAPI for nuclear staining.

Immunohistochemistry-Paraffin: RPS3A Antibody [NBP2-94503] - Paraffin-embedded rat brain using RPS3A .

Western Blot: RPS3A Antibody [NBP2-94503] - Analysis of extracts of various cell lines, using RPS3A at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit . Exposure time: 90s.

Immunohistochemistry-Paraffin: RPS3A Antibody [NBP2-94503] - Paraffin-embedded human liver cancer using RPS3A .

Immunohistochemistry-Paraffin: RPS3A Antibody [NBP2-94503] - Paraffin-embedded mouse brain using RPS3A .

• Reactivity: Hu, Mu, Rt
• Applications: WB, ICC/IF, IHC, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

RPS3A Antibody - BSA Free Summary

• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-264 of human RPS3A (NP_000997.1). MAVGKNKRLTKGGKKGAKKKVVDPFSKKDWYDVKAPAMFNIRNIGKTLVTRTQGTKIASDGLKGRVFEVSLADLQNDEVAFRKFKLITEDVQGKNCLTNFHGMDLTRDKMCSMVKKWQTMIEAHVDVKTTDGYLLRLFCVGFTKKRNNQIRKTSYAQHQQVRQIRKKMMEIMTREVQTNDLKEVVNKLIPDSIGKDIEKACQSIYPLHDVFVRKVKMLKKPKFELGKLMELHGEGSSSGKATGDETGAKVERADGYEPPVQESV
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: RPS3A
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:50-1:200
  • Immunohistochemistry 1:50-1:200
  • Immunohistochemistry-Paraffin
  • Immunoprecipitation 1:50-1:100
  • Western Blot 1:500-1:2000
• Theoretical MW: 29 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 50% glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Affinity purified

Alternate Names for RPS3A Antibody - BSA Free

• 40S ribosomal protein S3a
• FTE1
• fte-1
• MFTL
• MGC23240
• ribosomal protein S3A
• v-fos transformation effector protein 1
• v-fos transformation effector protein

Background

Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 40S subunit. The protein belongs to the S3AE family of ribosomal proteins. It is located in the cytoplasm. Disruption of the gene encoding rat ribosomal protein S3a, also named v-fos transformation effector protein, in v-fos-transformed rat cells results in reversion of the transformed phenotype. Transcript variants utilizing alternative transcription start sites have been described. This gene is co-transcribed with the U73A and U73B small nucleolar RNA genes, which are located in its fourth and third introns, respectively. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: RPS3A